LOCUS CR457271 1035 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834C0313D for
gene NAGK, N-acetylglucosamine kinase; complete cds, incl.
stopcodon.
ACCESSION CR457271
VERSION CR457271.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1035)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1035)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834C0313D, ORFNo 2248
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834C0313D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence BC001029 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1035
/db_xref="H-InvDB:HIT000268121"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834C0313D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1035
/codon_start=1
/gene="NAGK"
/db_xref="GOA:Q9UJ70"
/db_xref="H-InvDB:HIT000268121.14"
/db_xref="HGNC:HGNC:17174"
/db_xref="InterPro:IPR002731"
/db_xref="InterPro:IPR039758"
/db_xref="PDB:2CH5"
/db_xref="PDB:2CH6"
/db_xref="UniProtKB/Swiss-Prot:Q9UJ70"
/protein_id="CAG33552.1"
/translation="MAAIYGGVEGGGTRSEVLLVSEDGKILAEADGLSTNHWLIGTDK
CVERINEMVNRAKRKAGVDPLVPLRSLGLSLSGGDQEDAGRILIEELRDRFPYLSESY
LITTDAAGSIATATPDGGVVLISGTGSNCRLINPDGSESGCGGWGHMMGDEGSAYWIA
HQAVKIVFDSIDNLEAAPHDIGYVKQAMFHYFQVPDRLGILTHLYRDFDKCRFAGFCR
KIAEGAQQGDPLSRYIFRKAGEMLGRHIVAVLPEIDPVLFQGKIGLPILCVGSVWKSW
ELLKEGFLLALTQGREIQAQNFFSSFTLMKLRHSSALGGASLGARHIGHLLPMDYSAN
AIAFYSYTFS"
BASE COUNT 229 a 263 c 319 g 224 t
ORIGIN
1 atggccgcga tctatggggg tgtagagggg ggaggcacac gatccgaggt ccttttagtc
61 tcagaggatg ggaagatcct ggcagaagca gatggactga gcacaaacca ctggctgatc
121 gggacagaca agtgtgtgga gaggatcaat gagatggtga acagggccaa acggaaagcg
181 ggggtggatc ctctggtacc gctgcgaagc ttgggcctat ctctgagcgg tggggaccag
241 gaggacgcgg ggaggatcct gatcgaggag ctgagggacc gatttcccta cctgagtgaa
301 agctacttaa tcaccaccga tgccgccggc tccatcgcca cagctacacc ggatggtgga
361 gttgtgctca tatctggaac aggctccaac tgcaggctca tcaaccctga tggctccgag
421 agtggctgcg gcggctgggg ccatatgatg ggtgatgagg gttcagccta ctggatcgca
481 caccaagcag tgaaaatagt gtttgactcc attgacaacc tagaggcggc tcctcatgat
541 atcggctacg tcaaacaggc catgttccac tatttccagg tgccagatcg gctagggata
601 ctcactcacc tgtataggga ctttgataaa tgcaggtttg ctgggttttg ccggaaaatt
661 gcagaaggtg ctcagcaggg agaccccctt tcccgctata tcttcaggaa ggctggggag
721 atgctgggca gacacatcgt agcagtgttg cccgagattg acccggtctt gttccagggc
781 aagattggac tccccatcct gtgcgtgggc tctgtgtgga agagctggga gctgctgaag
841 gaaggttttc ttttggcgct gacccagggc agagagatcc aggctcagaa cttcttctcc
901 agcttcaccc tgatgaagct gaggcactcc tccgctctgg gtggggccag cctaggggcc
961 aggcacatcg ggcacctcct ccccatggac tatagcgcca atgccattgc cttctattcc
1021 tacacctttt cttaa
//