LOCUS CR457262 1794 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D036D for
gene FLJ10569, hypothetical protein FLJ10569; complete cds, incl.
stopcodon.
ACCESSION CR457262
VERSION CR457262.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1794)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1794)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834D036D, ORFNo 2228
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D036D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence BC051282 we found amino acid
exchange(s) at position (first base of changed triplet):
895(ser->asn)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1794
/db_xref="H-InvDB:HIT000268112"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834D036D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1794
/codon_start=1
/gene="FLJ10569"
/db_xref="GOA:Q9NVR2"
/db_xref="H-InvDB:HIT000268112.11"
/db_xref="HGNC:HGNC:25548"
/db_xref="InterPro:IPR026164"
/db_xref="UniProtKB/Swiss-Prot:Q9NVR2"
/protein_id="CAG33543.1"
/translation="MLLKVTEQCFNTLERSEMLLLLLRRFPETVVQHGVGLGEALLEA
ETIEEQESPVNCFRKLFVCDVLPLIINNHDVRLPANLLYKYLNKAAEFYINYVTRSTQ
IENQHQGAQDTSDLMSPSKRSSQKYIIEGLTEKSSQIVDPWERLFKILNVVGMRCEWQ
MDKGRRSYGDILHRMKDLCRYMNNFDSEAHAKYKNQVVYSTMLVFFKNAFQYVNSIQP
SLFQGPNAPSQVPLVLLEDVSNVYGDVEIDRNKHIHKKRKLAEGREKTMSSDDEDCSA
KGRNRHIVVNKAELANSTEVLENFKLARESWELLYSLEFLDKEFTRICLAWKTDTWLW
LRIFLTDMIIYQGQYKKAIASLHHLAALQGSISQPQITGQGTLEHQRALIQLATCHFA
LGEYRMTCEKVLDLMCYMVLPIQDGGKSQEEPSKVKPKFRKGSDLKLLPCTSKAIMPY
CLHLMLACFKLRAFTDNRDDMALGHVIVLLQQEWPRGENLFLKAVNKICQQGNFQYEN
FFNYVTNIDMLEEFAYLRTQEGGKIHLELLPNQGMLIKHHTVTRGITKGVKEDFRLAM
ERQVSRCGENLMVVLHRFCINEKILLLQTLT"
BASE COUNT 551 a 358 c 416 g 469 t
ORIGIN
1 atgttactaa aggtcacgga acaatgcttc aacacgttag aacgatcaga aatgttgctt
61 ctacttttga ggcgcttccc tgaaacggtg gtgcagcatg gggttggcct tggggaggca
121 ctattagagg ctgaaactat tgaagaacaa gaatctccag tgaactgctt tagaaaatta
181 tttgtttgtg atgtccttcc tctaataatt aacaaccatg atgttcgatt acctgccaat
241 ttattgtata agtacttgaa caaagcagct gaattttata tcaattatgt cactaggtct
301 actcaaatag aaaatcagca tcaaggcgcc caggatacat ctgatttaat gtcacctagc
361 aaacgtagct ctcagaagta cataatagaa gggctgacgg aaaaatcatc ccagatcgtg
421 gacccttggg agaggttgtt taagattttg aatgttgttg gaatgagatg tgaatggcag
481 atggataaag gaagacgaag ctatggagat attttgcata gaatgaagga tctctgcaga
541 tacatgaaca actttgatag tgaagcacat gcaaaatata aaaaccaagt ggtgtattcc
601 accatgctgg tcttctttaa gaatgcattc cagtatgtca acagcataca gccatctctc
661 ttccaaggtc ctaatgcccc gagccaagtt ccactggttc ttcttgaaga tgtatcgaat
721 gtgtatggtg atgtagaaat tgatcgtaat aaacacatcc ataaaaagag gaaactagct
781 gaaggaagag aaaaaaccat gagttcagac gatgaagact gttcggcgaa aggaagaaat
841 cgtcacattg tagtcaataa agccgaactt gctaactcca ctgaagtgtt agaaaacttt
901 aaattggcca gggagagctg ggagttgctc tattccctag aattccttga caaagaattt
961 acaaggattt gcttggcctg gaagacggat acttggcttt ggttaagaat cttcctcact
1021 gatatgatca tctatcaggg tcaatataaa aaggcgatag ccagcctgca tcacttagca
1081 gctctccagg gatccatttc tcagccacag atcacagggc aggggaccct ggagcatcag
1141 agggcgctca tccagctggc gacgtgccac tttgcgctag gggagtacag aatgacatgt
1201 gaaaaagtcc ttgatttgat gtgctacatg gtactcccca ttcaagatgg aggcaaatcc
1261 caggaggaac cctcgaaagt aaagcccaaa tttagaaaag gttcggatct gaagctcctg
1321 ccttgtacca gcaaggctat catgccatac tgcctccatt taatgttagc ctgttttaag
1381 cttagagctt tcacagacaa cagagacgac atggcattgg ggcatgtgat tgtgttgctt
1441 cagcaagagt ggccacgggg cgagaatctt ttcctgaaag ctgtcaataa aatttgccaa
1501 caaggaaatt tccaatatga gaattttttc aattacgtta caaatattga tatgctggag
1561 gaatttgcct acttgagaac tcaggaaggt gggaaaattc atctggaatt actacccaat
1621 caaggaatgc tgatcaagca ccacactgta actcgaggca tcaccaaagg cgtgaaggag
1681 gactttcgcc tggccatgga gcgccaggtc tcccgctgtg gagagaatct gatggtggtt
1741 ctgcacaggt tctgcattaa tgagaagatc ttgctccttc agactctgac ttaa
//