LOCUS CR457261 2481 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834H0221D for
gene ELAC2, elaC homolog 2 (E. coli); complete cds, incl.
stopcodon.
ACCESSION CR457261
VERSION CR457261.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 2481)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 2481)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834H0221D, ORFNo 2225
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834H0221D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_018127 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..2481
/db_xref="H-InvDB:HIT000268111"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834H0221D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..2481
/codon_start=1
/gene="ELAC2"
/db_xref="GOA:Q9BQ52"
/db_xref="H-InvDB:HIT000268111.11"
/db_xref="HGNC:HGNC:14198"
/db_xref="InterPro:IPR001279"
/db_xref="InterPro:IPR027794"
/db_xref="InterPro:IPR036866"
/db_xref="UniProtKB/Swiss-Prot:Q9BQ52"
/protein_id="CAG33542.1"
/translation="MWALCSLLRSAAGRTMSQGRTISQAPARRERPRKDPLRHLRTRE
KRGPSGCSGGPNTVYLQVVAAGSRDSGAALYVFSEFNRYLFNCGEGVQRLMQEHKLKV
ARLDNIFLTRMHWSNVGGLSGMILTLKETGLPKCVLSGPPQLEKYLEAIKIFSGPLKG
IELAVRPHSAPEYEDETMTVYQIPIHSEQRRGKHQPWQSPERPLSRLSPERSSDSELN
ENEPHLPHGVSQRRGVRDSSLVVAFICKLHLKRGNFLVLKAKEMGLPVGTAAIAPIIA
AVKDGKSITHEGREILAEELCTPPDPGAAFVVVECPDESFIQPICENATFQRYQGKAD
APVALVVHMAPASVLVDSRYQQWMERFGPDTQHLVLNENCASVHNLRSHKIQTQLNLI
HPDIFPLLTSFRCKKEGPTLSVPMVQGECLLKYQLRPRREWQRDAIITCNPEEFIVEA
LQLPNFQQSVQEYRRSAQDGPAPAEKRSQYPEIIFLGTGSAIPMKIRNVSATLVNISP
DTSLLLDCGEGTFGQLCRHYGDQVDRVLGTLAAVFVSHLHADHHTGLPSILLQRERAL
ASLGKPLHPLLVVAPNQLKAWLQQYHNQCQEVLHHISMIPAKCLQEGAEISSPAVERL
ISSLLRTCDLEEFQTCLVRHCKHAFGCALVHTSGWKVVYSGDTMPCEALVRMGKDATL
LIHEATLEDGLEEEAVEKTHSTTSQAISVGMRMNAEFIMLNHFSQRYAKVPLFSPNFS
EKVGVAFDHMKVCFGDFPTMPKLIPPLKALFAGDIEEMEERREKRELRQVRAALLSRE
LAGGLEDGEPQQKRAHTEEPQAKKVRAQ"
BASE COUNT 587 a 685 c 712 g 497 t
ORIGIN
1 atgtgggcgc tttgctcgct gctgcggtcc gcggccggac gcaccatgtc gcagggacgc
61 accatatcgc aggcacccgc ccgccgcgag cggccgcgca aggacccgct gcggcacctg
121 cgcacgcgag agaagcgcgg accgtcgggg tgctccggcg gcccaaacac cgtgtacctg
181 caggtggtgg cagcgggtag ccgggactcg ggcgccgcgc tctacgtctt ctccgagttc
241 aaccggtatc tcttcaactg tggagaaggc gttcagagac tcatgcagga gcacaagtta
301 aaggttgctc gcctggacaa catattcctg acacgaatgc actggtctaa tgttgggggc
361 ttaagtggaa tgattcttac tttaaaggaa accgggcttc caaagtgtgt actttctgga
421 cctccacaac tggaaaaata cctcgaagca atcaaaatat tttctggtcc attgaaagga
481 atagaactgg ctgtgcggcc ccactctgcc ccagaatacg aggatgaaac catgacagtt
541 taccagatcc ccatacacag tgaacagagg aggggaaagc accaaccatg gcagagtcca
601 gaaaggcctc tcagcaggct cagtccagag cgatcttcag actccgagtt gaatgaaaat
661 gagccacacc ttccacatgg tgttagccag agaagagggg tcagggactc ttccctggtc
721 gtagctttca tctgtaagct tcacttaaag agaggaaact tcttggtgct caaagcaaag
781 gagatgggcc tcccagttgg gacagctgcc atcgctccca tcattgctgc tgtcaaggac
841 gggaaaagca tcactcatga aggaagagag attttggctg aagagctgtg tactcctcca
901 gatcctggtg ctgcttttgt ggtggtagaa tgtccagatg aaagcttcat tcaacccatc
961 tgtgagaatg ccacctttca gaggtaccaa ggaaaggcag atgcccccgt ggccttggtg
1021 gttcacatgg ccccagcatc tgtgcttgtg gacagcaggt accagcagtg gatggagagg
1081 tttgggcctg acacccagca cttggtcctg aatgagaact gtgcctcagt tcacaacctt
1141 cgcagccaca agattcaaac ccagctcaac ctcatccacc cggacatctt ccccctgctc
1201 accagtttcc gctgtaagaa ggagggcccc accctcagtg tgcccatggt tcagggtgaa
1261 tgcctcctca agtaccagct ccgtcccagg agggagtggc agagggatgc cattattact
1321 tgcaatcctg aggaattcat agttgaggcg ctgcagcttc ccaacttcca gcagagcgtg
1381 caggagtaca ggaggagtgc gcaggacggc ccagccccag cagagaaaag aagtcagtac
1441 ccagaaatca tcttccttgg aacagggtct gccatcccga tgaagattcg aaatgtcagt
1501 gccacacttg tcaacataag ccccgacacg tctctgctac tggactgtgg tgagggcacg
1561 tttgggcagc tgtgccgtca ttacggagac caggtggaca gggtcctggg caccctggct
1621 gctgtgtttg tgtcccacct gcacgcagat caccacacgg gcttgccaag tatcttgctg
1681 cagagagaac gcgccttggc atctttggga aagccgcttc accctttgct ggtggttgcc
1741 cccaaccagc tcaaagcctg gctccagcag taccacaacc agtgccagga ggtcctgcac
1801 cacatcagta tgattcctgc caaatgcctt caggaagggg ctgagatctc cagtcctgca
1861 gtggaaagat tgatcagttc gctgttgcga acatgtgatt tggaagagtt tcagacctgt
1921 ctggtgcggc actgcaagca tgcgtttggc tgtgcgctgg tgcacacctc tggctggaaa
1981 gtggtctatt ccggggacac catgccctgc gaggctctgg tccggatggg gaaagatgcc
2041 accctcctga tacatgaagc caccctggaa gatggtttgg aagaggaagc agtggaaaag
2101 acacacagca caacgtccca agccatcagc gtggggatgc ggatgaacgc ggagttcatt
2161 atgctgaacc acttcagcca gcgctatgcc aaggtccccc tcttcagccc caacttcagc
2221 gagaaagtgg gagttgcctt tgaccacatg aaggtctgct ttggagactt tccaacaatg
2281 cccaagctga ttcccccact gaaagccctg tttgctggcg acatcgagga gatggaggag
2341 cgcagggaga agcgggagct gcggcaggtg cgggcggccc tcctgtccag ggagctggca
2401 ggcggcctgg aggatgggga gcctcagcag aagcgggccc acacagagga gccacaggcc
2461 aagaaggtca gagcccagtg a
//