LOCUS CR457254 756 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D109D for
gene FLJ10350, hypothetical protein FLJ10350; complete cds, incl.
stopcodon.
ACCESSION CR457254
VERSION CR457254.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 756)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 756)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834D109D, ORFNo 2208
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D109D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence AK001212 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..756
/db_xref="H-InvDB:HIT000268104"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834D109D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..756
/codon_start=1
/gene="FLJ10350"
/db_xref="GOA:Q3KQU3"
/db_xref="H-InvDB:HIT000268104.13"
/db_xref="HGNC:HGNC:25514"
/db_xref="InterPro:IPR008604"
/db_xref="UniProtKB/Swiss-Prot:Q3KQU3"
/protein_id="CAG33535.1"
/translation="MAGTTDREEATRLLAEKRRQAREQREREEQERRLQAERDKRMRE
EQLAREAEARAEREAEARRREEQEAREKAQAEQEEQERLQKQKEEAEARSREEAERQR
LEREKHFQQQEQERQERRKRLEEIMKRTRKSEVSETKKQDSKEANANGSSPEPVKAVE
ARSPGLQKEAVQKEEPIPQEPQWSLPSKELPASLVNGLQPLPAHQENGFSTNGPSGDK
SLSRTPETLLPFAEAEAFLKKAVVQSPQVTEVL"
BASE COUNT 200 a 199 c 281 g 76 t
ORIGIN
1 atggccggca ccacagaccg agaagaagcc actcggctct tggctgagaa gcggcgccag
61 gcccgggagc agcgggagcg cgaggagcag gagcggaggc tgcaggcaga aagggacaag
121 cgaatgcgag aggagcagct ggcacgggag gccgaggccc gggcggagcg ggaggcggag
181 gcccggaggc gggaggagca ggaggcacga gagaaggcgc aggccgagca ggaggagcag
241 gagcggctgc agaagcagaa agaggaggcc gaagctcggt cgcgggaaga ggcggagcgg
301 cagcgtctgg agcgggaaaa gcacttccag cagcaggagc aagagcggca agagcgcaga
361 aagcgtctgg aggagatcat gaagaggact cggaagtcag aagtttctga aaccaagaag
421 caggacagca aggaggccaa cgccaacggt tccagcccag agcctgtgaa agctgtggag
481 gctcggtccc cagggctgca gaaggaggct gtgcagaaag aggagcccat cccacaggag
541 cctcagtgga gtctcccaag caaggagttg ccagcgtccc tggtgaatgg cctgcagcct
601 ctcccagcac accaggagaa tggcttctcc accaacggac cctctgggga caagagtctg
661 agccgaacac cagagacact cctgcccttt gcagaggcag aagccttcct caagaaagct
721 gtggtgcagt ccccgcaggt cacagaagtc ctttaa
//