LOCUS CR457250 486 bp mRNA linear HUM 03-JUN-2004
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834C099D for
gene FLJ20847, hypothetical protein FLJ20847; complete cds, incl.
stopcodon.
ACCESSION CR457250
VERSION CR457250.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 486)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 486)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834C099D, ORFNo 2201
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834C099D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence AK000854 we found amino acid
exchange(s) at position (first base of changed triplet):
325(phe->ser) 481(met->ile)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..486
/db_xref="H-InvDB:HIT000268100"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834C099D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..486
/codon_start=1
/gene="FLJ20847"
/db_xref="H-InvDB:HIT000268100.12"
/db_xref="UniProtKB/TrEMBL:Q6IAA5"
/protein_id="CAG33531.1"
/translation="MSYLSGSSASPARRLGVVKVVPRPCLQWLENPGGCLGPSGQREA
GSSSPGDCGHIGACLGLEGQVTSPATLPSLLWGPHFRATLPEAHASIHSFSALNLIHK
QPPPFPSPSHSVDVILPPPVSILRQASKEALTLLPKWCFLKNTITTLGAIFSHLPVFR
I"
BASE COUNT 97 a 155 c 111 g 123 t
ORIGIN
1 atgtcctatt tgagtggcag cagcgccagc ccagcaagaa ggctgggggt tgtcaaggtt
61 gtccccagac cttgcttgca gtggttggag aacccagggg gctgccttgg gccctctggc
121 cagagggaag cgggcagctc tagccctgga gattgtggtc acattggggc ttgtttagga
181 ttggagggcc aggtcacctc cccagccacc ctcccttctc tcctctgggg tccccacttt
241 agggcgactt tgcccgaggc ccacgcatcc atccactcct ttagtgcctt gaatcttatt
301 cacaagcagc cccctccctt cccctcccct tctcactctg ttgatgtaat cctcccaccc
361 ccagtgtcca tcctaagaca ggcatcaaaa gaggccctaa ctttacttcc caaatggtgc
421 tttttaaaaa acaccatcac tacattaggg gcaatttttt cacacctccc tgtcttcaga
481 atttaa
//