LOCUS CR457249 918 bp mRNA linear HUM 17-APR-2005
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834H0912D for
gene FBXO34, F-box only protein 34; complete cds, incl. stopcodon.
ACCESSION CR457249
VERSION CR457249.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 918)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 918)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834H0912D, ORFNo 2195
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834H0912D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence AK000732 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..918
/db_xref="H-InvDB:HIT000268099"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834H0912D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..918
/codon_start=1
/gene="FBXO34"
/db_xref="GOA:Q9NWN3"
/db_xref="H-InvDB:HIT000268099.13"
/db_xref="HGNC:HGNC:20201"
/db_xref="InterPro:IPR001810"
/db_xref="InterPro:IPR036047"
/db_xref="InterPro:IPR039594"
/db_xref="UniProtKB/Swiss-Prot:Q9NWN3"
/protein_id="CAG33530.1"
/translation="MTDELVGLPFSSHTYSQASELPTDAVDCMSRELVSLTSRNPDQR
KESLCISITVSKVDKDQPSNLNSCEDPVPGMLFFLPPGQHLSDYSQLNESTTKESSEA
SQLEDAAGGDSASEEKSGSAEPFVPPASSVESTLPVLEASSWKKQVSHDFLETRFKIQ
QLLEPQQYMAFLPHHIMVKIFRLLPTKSLVALKCTCCYFKFIIEYYNIRPADSRWVRD
PRYREDPCKQCKKKYVKGDVSLCRWHPKPYCQALPYGPGYWMCCHRSQKGFPGCKLGL
HDNHWVPACHSFNRAIHKKAKGTEAEEEY"
BASE COUNT 244 a 215 c 220 g 239 t
ORIGIN
1 atgacagatg aactcgttgg gttacctttt tcctctcata cctattccca agcctctgaa
61 ttgcccacag atgctgttga ttgtatgagc agagagcttg tgtcccttac tagccgaaat
121 cctgatcaaa gaaaagaatc tttgtgcatt agtatcactg tgtccaaggt agacaaagac
181 cagccttcca atttaaactc ctgtgaagac ccagttccag ggatgttgtt ttttttgcca
241 cctggtcagc acttgtcaga ctattcccag ttgaatgaaa gcacaacaaa agagtcttca
301 gaggccagcc agcttgaaga tgctgctggg ggtgacagtg catctgagga aaaaagtggg
361 tctgctgagc catttgtacc gccagcctct tctgtggaaa gtacattacc agtgcttgag
421 gcatccagtt ggaagaagca ggtgtcgcat gacttcctgg agaccaggtt taaaatccag
481 cagcttttgg agcctcagca gtacatggct tttctgcccc accacattat ggtaaaaatc
541 ttcaggttac ttcccaccaa gagtttagtg gcccttaaat gtacctgctg ctatttcaag
601 tttatcattg agtactacaa tatcaggcca gcagattctc gctgggttcg agatccacgc
661 tatagagagg atccttgcaa acagtgcaag aaaaagtatg tgaaagggga tgtgtccctg
721 tgccgatggc accccaagcc ctattgccag gcattgccct atgggccagg gtattggatg
781 tgctgccacc ggtctcagaa aggattccct ggctgtaagc tggggcttca tgacaatcac
841 tgggttcctg cctgccacag ctttaatcgg gcaatccata agaaagcaaa agggactgaa
901 gctgaagagg aatattaa
//