LOCUS CR457242 915 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834H0812D for
gene FLJ20551, hypothetical protein FLJ20551; complete cds, incl.
stopcodon.
ACCESSION CR457242
VERSION CR457242.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 915)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 915)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834H0812D, ORFNo 2183
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834H0812D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence BC013194 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..915
/db_xref="H-InvDB:HIT000268092"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834H0812D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..915
/codon_start=1
/gene="FLJ20551"
/db_xref="GOA:Q96DW6"
/db_xref="H-InvDB:HIT000268092.13"
/db_xref="HGNC:HGNC:26054"
/db_xref="InterPro:IPR018108"
/db_xref="InterPro:IPR023395"
/db_xref="InterPro:IPR030847"
/db_xref="UniProtKB/Swiss-Prot:Q96DW6"
/protein_id="CAG33523.1"
/translation="MIQNSRPSLLQPQDVGDTVETLMLHPVIKAFLCGSISGTCSTLL
FQPLDLLKTRLQTLQPSDHGSRRVGMLAVLLKVVRTESLLGLWKGMSPSIVRCVPGVG
IYFGTLYSLKQYFLRGHPPTALESVMLGVGSRSVAGVCMSPITVIKTRYESGKYGYES
IYAALRSIYHSEGHRGLFSGLTATLLRDAPFSGIYLMFYNQTKNIVPHDQVDATLIPI
TNFSCGIFAGILASLVTQPADVIKTHMQLYPLKFQWIGQAVTLIFKDYGLRGFFQGGI
PRALRRTLMAAMAWTVYEEMMAKMGLKS"
BASE COUNT 204 a 236 c 229 g 246 t
ORIGIN
1 atgattcaga actcacgtcc gtcgctgctg caaccccaag atgtcggaga cacggtggaa
61 acgcttatgt tacatccggt gatcaaggct ttcctgtgtg gctccatcag tgggacctgc
121 tctaccctcc ttttccaacc tctggatctc cttaaaacac gcctgcaaac cctccagccc
181 tcagatcatg ggtctagacg tgttgggatg ttggctgtac tcttgaaggt ggttcgcacg
241 gagagtcttt tgggcctttg gaaagggatg tccccttcca ttgtgagatg tgtccctggc
301 gttggaatct actttggcac tctctactct ttgaagcagt atttcttgcg aggccatccc
361 ccaaccgccc tggagtcagt catgctgggg gtgggctctc gctctgttgc aggggtctgt
421 atgtcaccta tcactgtaat caagacgcgc tatgagagtg ggaaatatgg ctatgagagt
481 atctacgctg ccctgaggag catctatcac agtgaggggc accggggcct cttcagtggc
541 ctgacagcaa ctctccttcg agatgcgccc ttctcaggaa tctacctgat gttttacaac
601 cagaccaaaa atatagtgcc tcatgaccag gtggatgcaa cccttattcc tattacaaat
661 ttcagctgtg ggatatttgc tggtattctg gcctcactgg taactcaacc tgcggatgtt
721 atcaaaactc atatgcagct ttatccactg aagtttcaat ggattggcca agcagtgaca
781 cttattttca aagactatgg actacgtggc ttcttccaag gtggcatccc ccgagccctc
841 cgcagaactc taatggcagc aatggcgtgg acggtgtatg aagagatgat ggccaagatg
901 ggcctgaagt cttaa
//