LOCUS CR457234 747 bp mRNA linear HUM 03-JUN-2004
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D049D for
gene FLJ20647, hypothetical protein FLJ20647; complete cds, incl.
stopcodon.
ACCESSION CR457234
VERSION CR457234.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 747)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 747)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834D049D, ORFNo 2158
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D049D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_017918 we found amino acid
exchange(s) at position (first base of changed triplet):
313(asp->gly) 376(glu->gly) 499(ile->val)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..747
/db_xref="H-InvDB:HIT000268084"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834D049D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..747
/codon_start=1
/gene="FLJ20647"
/db_xref="GOA:Q9NWR8"
/db_xref="H-InvDB:HIT000268084.13"
/db_xref="HGNC:HGNC:26076"
/db_xref="InterPro:IPR006769"
/db_xref="InterPro:IPR039055"
/db_xref="UniProtKB/Swiss-Prot:Q9NWR8"
/protein_id="CAG33515.1"
/translation="MLSTVGSFLQDLQNEDKGIKTAAIFTADGNMISASTLMDILLMN
DFKLVINKIAYDVQCPKREKPSNEHTAEMEHMKSLVHRLFTILHLEESQKKREHHLLE
KIGHLKEQLQPLEQVKAGIEAHSGAKTSGLLWAGLALLSIQGGALAWLTWWVYSWDIM
EPVTYFVTFANSMVFFAYFIVTRQDYTYSAVKSRQFLQFFHKKSKQQHFDVQQYNKLK
EDLAKAKESLKQARHSLCLQMQVEELNEKN"
BASE COUNT 239 a 150 c 162 g 196 t
ORIGIN
1 atgttgtcaa cagttggttc attccttcag gacctacaaa atgaagataa gggtatcaaa
61 actgcagcca tcttcacagc agatggcaac atgatttcag cttctacctt gatggatatt
121 ttgctaatga atgattttaa acttgtcatt aataaaatag catatgatgt gcagtgtcca
181 aagagagaaa aaccaagtaa tgagcacact gctgagatgg aacacatgaa atccttggtt
241 cacagactat ttacaatctt gcatttagaa gagtctcaga aaaagagaga gcaccattta
301 ctggagaaaa ttggccacct gaaggaacag ctgcagcccc ttgaacaggt gaaagctgga
361 atagaagctc attcgggagc caaaaccagt ggactcctgt gggctggatt ggcactgctg
421 tccattcagg gtggggcact ggcctggctc acgtggtggg tgtactcctg ggatatcatg
481 gagccagtta catacttcgt cacatttgca aattctatgg tcttttttgc atactttata
541 gtcactcgac aggattatac ttactcagct gttaagagta ggcaatttct tcagttcttc
601 cacaagaaat caaagcaaca gcactttgat gtgcagcaat acaacaagtt aaaagaagac
661 cttgctaagg ctaaagaatc cctgaaacag gcgcgccatt ctctctgttt gcaaatgcaa
721 gtagaagaac tcaatgaaaa gaattaa
//