LOCUS CR457219 216 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E0616D for
gene UBE2Q, ubiquitin-conjugating enzyme E2Q (putative); complete
cds, incl. stopcodon.
ACCESSION CR457219
VERSION CR457219.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 216)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 216)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834E0616D, ORFNo 2101
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E0616D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence BC015316 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..216
/db_xref="H-InvDB:HIT000268069"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834E0616D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..216
/codon_start=1
/gene="UBE2Q"
/db_xref="GOA:Q7Z7E8"
/db_xref="H-InvDB:HIT000268069.13"
/db_xref="HGNC:HGNC:15698"
/db_xref="InterPro:IPR000608"
/db_xref="InterPro:IPR016135"
/db_xref="PDB:2QGX"
/db_xref="UniProtKB/Swiss-Prot:Q7Z7E8"
/protein_id="CAG33500.1"
/translation="MELLTKQGWSSAYSIESVIMQISATLVKGKARVQFGANKSQYSL
TRAQQSYKSLVQIHEKNGWYTPPKEDG"
BASE COUNT 72 a 54 c 53 g 37 t
ORIGIN
1 atggaacttc tcaccaaaca gggctggagc agtgcctact ccatagagtc agtgatcatg
61 cagatcagtg ccacactggt gaaggggaaa gcacgagtgc agtttggagc caacaaatct
121 caatacagtc tgacaagagc acagcagtcc tacaagtcct tggtgcagat ccacgaaaaa
181 aacggctggt acacaccccc aaaagaagac ggttaa
//