LOCUS CR457191 912 bp mRNA linear HUM 03-JUN-2004
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D1212D for
gene MRPL1, mitochondrial ribosomal protein L1; complete cds, incl.
stopcodon.
ACCESSION CR457191
VERSION CR457191.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 912)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 912)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834D1212D, ORFNo 2038
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D1212D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_020236 we found amino acid
exchange(s) at position (first base of changed triplet):
619(asp->his)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..912
/db_xref="H-InvDB:HIT000268041"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834D1212D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..912
/codon_start=1
/gene="MRPL1"
/db_xref="GOA:Q9BYD6"
/db_xref="H-InvDB:HIT000268041.11"
/db_xref="HGNC:HGNC:14275"
/db_xref="InterPro:IPR005879"
/db_xref="InterPro:IPR016095"
/db_xref="InterPro:IPR023674"
/db_xref="InterPro:IPR028364"
/db_xref="UniProtKB/Swiss-Prot:Q9BYD6"
/protein_id="CAG33472.1"
/translation="MVYQTSLCSCSVNIRVPNRHFAAATKSAKKTKKGAKEKTPDEKK
DEIEKIKAYPYMEGEPEDDVYLKRLYPRQIYEVEKAVHLLKKFQILDFTSPKQSVYLD
LTLDMALGKKKNVEPFTSVLSLPYPFASEINKVAVFTENASEVKIAEENGAAFAGGTS
LIQKIWDDEIVADFYVAVPEIMPELNRLRKKLNKKYPKLSRNSIGRHIPKMLELFKNG
HEIKVDEERENFLQTKIATLDMSSDQIAANLQAVINEVCRHRPLNLGPFVVRAFLRSS
TSEGLLLKIDPLLPKEVKNEESEKEDA"
BASE COUNT 330 a 149 c 189 g 244 t
ORIGIN
1 atggtttatc agacatcact ttgttcttgt tctgtaaaca tccgagtgcc caacagacat
61 tttgctgctg ctacaaagtc tgcaaagaaa acaaaaaaag gtgctaaaga aaaaacacca
121 gatgagaaaa aagatgaaat agaaaaaata aaagcatatc cctatatgga aggcgaacct
181 gaggatgatg tctatttaaa acgcttatac ccgagacaga tatatgaggt ggagaaagct
241 gttcacttac ttaagaaatt tcaaattctt gactttacta gtccaaagca aagtgtttat
301 cttgatttga cactggatat ggcactggga aagaagaaaa acgtggagcc atttaccagt
361 gttcttagtt tgccataccc atttgcttcc gaaatcaata aagttgctgt atttacagag
421 aatgcatcag aggtcaaaat agcggaagaa aatggagctg catttgcagg aggcactagt
481 ctgatacaga agatttggga tgatgaaatt gttgcagact tttacgtagc tgttccagaa
541 ataatgcctg aacttaatcg attaaggaag aaactgaata aaaaatatcc aaagctttct
601 cgaaattcca ttggccgtca catccccaaa atgcttgaat tatttaaaaa tggacatgaa
661 attaaggtag atgaagaaag ggagaacttt ctccagacca aaatagcaac attggatatg
721 tcaagtgacc agatagctgc caatctgcaa gcagttatta atgaagtttg taggcacaga
781 ccgctgaatt tgggtccctt tgtggtacgt gctttccttc gtagttcaac aagtgaaggt
841 ttattactga agattgatcc attgttgcct aaagaagtaa aaaatgaaga aagtgaaaaa
901 gaagatgctt aa
//