LOCUS CR457186 558 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834H038D for
gene Pfs2, DNA replication complex GINS protein PSF2; complete cds,
incl. stopcodon.
ACCESSION CR457186
VERSION CR457186.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 558)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 558)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834H038D, ORFNo 2022
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834H038D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence BC003186 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..558
/db_xref="H-InvDB:HIT000268036"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834H038D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..558
/codon_start=1
/gene="Pfs2"
/db_xref="GOA:Q9Y248"
/db_xref="H-InvDB:HIT000268036.11"
/db_xref="HGNC:HGNC:24575"
/db_xref="InterPro:IPR007257"
/db_xref="InterPro:IPR021151"
/db_xref="InterPro:IPR036224"
/db_xref="PDB:2E9X"
/db_xref="PDB:2EHO"
/db_xref="PDB:2Q9Q"
/db_xref="UniProtKB/Swiss-Prot:Q9Y248"
/protein_id="CAG33467.1"
/translation="MDAAEVEFLAEKELVTIIPNFSLDKIYLIGGDLGPFNPGLPVEV
PLWLAINLKQRQKCRLLPPEWMDVEKLEKMRDHERKEETFTPMPSPYYMELTKLLLNH
ASDNIPKADEIRTLVKDMWDTRIAKLRVSADSFVRQQEAHAKLDNLTLMEINTSGTFL
TQALNHMYKLRTNLQPLESTQSQDF"
BASE COUNT 156 a 148 c 137 g 117 t
ORIGIN
1 atggacgctg ccgaggtcga attcctcgcc gagaaggagc tggttaccat tatccccaac
61 ttcagtctgg acaagatcta cctcatcggg ggggacctgg ggccttttaa ccctggttta
121 cccgtggaag tgcccctgtg gctggcgatt aacctgaaac aaagacagaa atgtcgcctg
181 ctccctccag agtggatgga tgtagaaaag ttggagaaga tgagggatca tgaacgaaag
241 gaagaaactt ttaccccaat gcccagccct tactacatgg aacttacgaa gctcctgtta
301 aatcatgctt cagacaacat cccgaaggca gacgaaatcc ggaccctggt caaggatatg
361 tgggacactc gtatagccaa actccgagtg tctgctgaca gctttgtgag acagcaggag
421 gcacatgcca agctggataa cttgaccttg atggagatca acaccagcgg gactttcctc
481 acacaagcgc tcaaccacat gtacaaactc cgcacaaacc tccagcctct ggagagtact
541 cagtctcagg acttttaa
//