LOCUS CR457180 957 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D0912D for
gene RDH11, retinol dehydrogenase 11 (all-trans and 9-cis);
complete cds, incl. stopcodon.
ACCESSION CR457180
VERSION CR457180.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 957)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 957)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834D0912D, ORFNo 2006
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D0912D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence AF151840 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..957
/db_xref="H-InvDB:HIT000268030"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834D0912D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..957
/codon_start=1
/gene="RDH11"
/db_xref="GOA:Q8TC12"
/db_xref="H-InvDB:HIT000268030.12"
/db_xref="HGNC:HGNC:17964"
/db_xref="InterPro:IPR002347"
/db_xref="InterPro:IPR036291"
/db_xref="UniProtKB/Swiss-Prot:Q8TC12"
/protein_id="CAG33461.1"
/translation="MVELMFPLLLLLLPFLLYMAAPQIRKMLSSGVCTSTVQLPGKVV
VVTGANTGIGKETAKELAQRGARVYLACRDVEKGELVAKEIQTTTGNQQVLVRKLDLS
DTKSIRAFAKGFLAEEKHLHVLINNAGVMMCPYSKTADGFEMHIGVNHLGHFLLTHLL
LEKLKESAPSRIVNVSSLAHHLGRIHFHNLQGEKFYNAGLAYCHSKLANILFTQELAR
RLKGSGVTTYSVHPGTVQSELVRHSSFMRWMWWLFSFFIKTPQQGAQTSLHCALTEGL
EILSGNHFSDCHVAWVSAQARNETIARRLWDVSCDLLGLPID"
BASE COUNT 238 a 234 c 250 g 235 t
ORIGIN
1 atggttgagc tcatgttccc gctgttgctc ctccttctgc ccttccttct gtatatggct
61 gcgccccaaa tcaggaaaat gctgtccagt ggggtgtgta catcaactgt tcagcttcct
121 gggaaagtag ttgtggtcac aggagctaat acaggtatcg ggaaggagac agccaaagag
181 ctggctcaga gaggagctcg agtatattta gcttgccggg atgtggaaaa gggggaattg
241 gtggccaaag agatccagac cacgacaggg aaccagcagg tgttggtgcg gaaactggac
301 ctgtctgata ctaagtctat tcgagctttt gctaagggct tcttagctga ggaaaagcac
361 ctccacgttt tgatcaacaa tgcaggagtg atgatgtgtc cgtactcgaa gacagcagat
421 ggctttgaga tgcacatagg agtcaaccac ttgggtcact tcctcctaac ccatctgctg
481 ctagagaaac taaaggaatc agccccatca aggatagtaa atgtgtcttc cctcgcacat
541 cacctgggaa ggatccactt ccataacttg cagggcgaga aattctacaa tgcaggcctg
601 gcctactgtc acagcaagct agccaacatc ctcttcaccc aggaactggc ccggagacta
661 aaaggctctg gcgttacgac gtattctgta caccctggca cagtccaatc tgaactggtt
721 cggcactcat ctttcatgag atggatgtgg tggcttttct cctttttcat caagactcct
781 cagcagggag cccagaccag cctgcactgt gccttaacag aaggtcttga gattctaagt
841 gggaatcatt tcagtgactg tcatgtggca tgggtctctg cccaagctcg taatgagact
901 atagcaaggc ggctgtggga cgtcagttgt gacctgctgg gcctcccaat agattaa
//