LOCUS CR457168 1122 bp mRNA linear HUM 03-JUN-2004
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D1012D for
gene MAGEE1, melanoma antigen, family E, 1, cancer/testis specific;
complete cds, incl. stopcodon.
ACCESSION CR457168
VERSION CR457168.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1122)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1122)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834D1012D, ORFNo 1978
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D1012D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_016249 we found amino acid
exchange(s) at position (first base of changed triplet):
1117(glu->asp)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1122
/db_xref="H-InvDB:HIT000268018"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834D1012D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1122
/codon_start=1
/gene="MAGEE1"
/db_xref="H-InvDB:HIT000268018.11"
/db_xref="InterPro:IPR002190"
/db_xref="InterPro:IPR037445"
/db_xref="InterPro:IPR041898"
/db_xref="InterPro:IPR041899"
/db_xref="UniProtKB/TrEMBL:Q6IAI7"
/protein_id="CAG33449.1"
/translation="MPPVPGVPFRNVDNDSPTSVELEDWVDAQHPTDEEEEEASSASS
TLYLVFSPSSFSTSSSLILGGPEEEEVPSGVIPNLTESIPSSPPQGPPQGPSQSPLSS
CCSSFSWSSFSEESSSQKGEDTGTCQGLPDSESSFTYTLDEKVAELVEFLLLKYEAEE
PVTEAEMLMIVIKYKDYFPVILKRAREFMELLFGLALIEVGPDHFCVFANTVGLTDEG
SDDEGMPENSLLIIILSVIFIKGNCASEEVIWEVLNAVGVYAGREHFVYGEPRELLTK
VWVQGHYLEYREVPHSSPPYYEFLWGPRAHSESIKKKVLEFLAKLNNTVPSSFPSWYK
DALKDVEERVQATIDTADDATVMASESLSVMSSNVSFSD"
BASE COUNT 268 a 279 c 289 g 286 t
ORIGIN
1 atgcctcccg ttccaggcgt tccattccgc aacgttgaca acgactcccc gacctcagtt
61 gagttagaag actgggtaga tgcacagcat cccacagatg aggaagagga ggaagcctcc
121 tccgcctctt ccactttgta cttagtattt tccccctctt ctttctccac atcctcttct
181 ctgattcttg gtggtcctga ggaggaggag gtgccctctg gtgtgatacc aaatcttacc
241 gagagcattc ccagtagtcc tccacagggt cctccacagg gtccttccca gagtcctctg
301 agctcctgct gctcctcttt ttcatggagc tcattcagtg aggagtccag cagccagaaa
361 ggggaggata caggcacctg tcagggcctg ccagacagtg agtcctcttt cacatataca
421 ctagatgaaa aggtggccga gttagtggag ttcctgctcc tcaaatacga agcagaggag
481 cctgtaacag aggcagagat gctgatgatt gtcatcaagt acaaagatta ctttcctgtg
541 atactcaaga gagcccgtga gttcatggag cttctttttg gccttgccct gatagaagtg
601 ggccctgacc acttctgtgt gtttgcaaac acagtaggcc tcaccgatga gggtagtgat
661 gatgagggca tgcccgagaa cagcctcctg attattattc tgagtgtgat cttcataaag
721 ggcaactgtg cctctgagga ggtcatctgg gaagtgctga atgcagtagg ggtatatgct
781 gggagggagc acttcgtcta tggggagcct agggagctcc tcactaaagt ttgggtgcag
841 ggacattacc tggagtatcg ggaggtgccc cacagttctc ctccatatta tgaattcctg
901 tggggtccaa gagcccattc agaaagcatc aagaagaaag tactagagtt tttagccaag
961 ctgaacaaca ctgttcctag ttcctttcca tcctggtaca aggatgcttt gaaagatgtg
1021 gaagagagag tccaggccac aattgatacc gcagatgatg ccactgtcat ggccagtgaa
1081 agcctcagtg tcatgtccag caacgtctcc ttttctgatt aa
//