LOCUS CR457158 858 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B0712D for
gene C3orf1, chromosome 3 open reading frame 1; complete cds, incl.
stopcodon.
ACCESSION CR457158
VERSION CR457158.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 858)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 858)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B0712D, ORFNo 1954
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B0712D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence AL390077 we found amino acid
exchange(s) at position (first base of changed triplet):
466(leu->pro)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..858
/db_xref="H-InvDB:HIT000268008"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B0712D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..858
/codon_start=1
/gene="C3orf1"
/db_xref="GOA:Q9NPL8"
/db_xref="H-InvDB:HIT000268008.12"
/db_xref="HGNC:HGNC:1321"
/db_xref="UniProtKB/Swiss-Prot:Q9NPL8"
/protein_id="CAG33439.1"
/translation="MEVPPPAPRSFLCRALCLFPRVFAAEAVTADSEVLEERQKRLPY
VPEPYYPESGWDRLRELFGKDEQQRISKDLADICKTAATAGIIGWVYGGIPAFIHAKQ
QYIEQSQAEIYHNRFDAVQSAHRAATRGFIRYGWRWGWRTAVFVTIFNTVNTSPNVYR
NKDALSHFVIAGAVTGSLFRINVGLRGLVAGGIIGALLGTPVGGLLMAFQKYSGETIQ
ERKQKDRKALHELKLEEWKGRLQVTEHLPEKIESSLQEDEPENDAKKIEALLNLPRNP
SVIDKQDKD"
BASE COUNT 241 a 185 c 229 g 203 t
ORIGIN
1 atggaggtgc cgccaccggc accgcggagc tttctctgta gagcattgtg cctatttccc
61 cgagtctttg ctgccgaagc tgtgactgcc gattcggaag tccttgagga gcgtcagaag
121 cggcttccct acgtcccaga gccctattac ccggaatctg gatgggaccg cctccgggag
181 ctgtttggca aagatgaaca gcagagaatt tcaaaggacc ttgctgatat ctgtaagacg
241 gcagctacag caggcatcat tggctgggtg tatgggggaa taccagcttt tattcatgct
301 aaacaacaat acattgagca gagccaggca gaaatttatc ataaccggtt tgatgctgtg
361 caatctgcac atcgtgctgc cacacgaggc ttcattcgtt atggctggcg ctggggttgg
421 agaactgcag tgtttgtgac tatattcaac acagtgaaca ctagtccgaa tgtataccga
481 aataaagatg ccttaagcca ttttgtaatt gcaggagctg tcacgggaag tctttttagg
541 ataaacgtag gcctgcgtgg cctggtggct ggtggcataa ttggagccct gctgggcact
601 cctgtaggag gcctgctgat ggcatttcag aagtactctg gtgagactat tcaggaaaga
661 aaacagaagg atcgaaaggc actccatgag ctaaaactgg aagagtggaa aggcagacta
721 caagttactg agcacctccc tgagaaaatt gaaagtagtt tacaggaaga tgaacctgag
781 aatgatgcta agaaaattga agcactgcta aaccttccta gaaacccttc agtaatagat
841 aaacaagaca aggattaa
//