LOCUS CR457153 1428 bp mRNA linear HUM 03-JUN-2004
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D0512D for
gene SPG3A, spastic paraplegia 3A (autosomal dominant); complete
cds, incl. stopcodon.
ACCESSION CR457153
VERSION CR457153.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1428)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1428)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834D0512D, ORFNo 1945
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D0512D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence AY032844 we found amino acid
exchange(s) at position (first base of changed triplet):
907(asp->gly) 1423(met->ile)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1428
/db_xref="H-InvDB:HIT000268003"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834D0512D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1428
/codon_start=1
/gene="SPG3A"
/db_xref="GOA:Q6IAK1"
/db_xref="H-InvDB:HIT000268003.12"
/db_xref="InterPro:IPR015894"
/db_xref="InterPro:IPR027417"
/db_xref="InterPro:IPR030386"
/db_xref="InterPro:IPR036543"
/db_xref="UniProtKB/TrEMBL:Q6IAK1"
/protein_id="CAG33434.1"
/translation="MDFMLRYMYNQESVDWVGDYNEPLTGFSWRGGSERETTGIQIWS
EIFLINKPDGKKVAVLLMDTQGTFDSQSTLRDSATVFALSTMISSIQVYNLSQNVQED
DLQHLQLFTEYGRLAMEETFLKPFQSLIFLVRDWSFPYEFSYGADGGAKFLEKRLKVS
GNQHEELQNVRKHIHSCFTNISCFLLPHPGLKVATNPNFDGKLKEIDDEFIKNLKILI
PWLLSPESLDIKEINGNKITCRGLVEYFKAYIKIYQGEELPHPKSMLQATAEANNLAA
VATAKDTYNKKMEEICGGDKPFLAPNGLQTKHLQLKEESVKLFRGVKKMGGEEFSRRY
LQQLESEIDELYIQYIKHNDSKNIFHAARTPATLFVVIFITYVIAGVTGFIGLDIIAS
LCNMIMGLTLITLCTWAYIRYSGEYRELGAVIDQVAAALWDQGSTNEALYKLYSAAAT
HRHLYHQAFPTPKSESTEQSEKKKI"
BASE COUNT 455 a 293 c 316 g 364 t
ORIGIN
1 atggacttca tgttgagata catgtacaac caggaatcag ttgattgggt tggagactac
61 aatgaaccat tgactggttt ttcatggaga ggtggatctg agcgagagac cacaggaatt
121 cagatatgga gtgaaatctt ccttatcaat aaacctgatg gtaaaaaggt tgcagtgtta
181 ttgatggata ctcagggaac ctttgatagt cagtcaactt tgagagattc agccacagta
241 tttgccctta gcacaatgat cagctcaata caggtatata acttatccca aaatgtccag
301 gaggatgatc ttcagcacct ccagcttttc actgagtatg gcagactggc aatggaggaa
361 acattcctga agccatttca gagtctgata tttcttgttc gagactggag tttcccatac
421 gaattttcat atggagccga tggtggtgcc aaattcttgg aaaaacgcct caaggtctca
481 gggaaccagc atgaagaact acagaacgtc agaaaacaca tccattcctg tttcaccaac
541 atttcctgtt ttctgctacc tcatcctggc ttaaaagtag ctaccaatcc aaactttgat
601 ggaaaattga aagaaataga tgatgaattc atcaaaaact tgaaaatact gattccttgg
661 ctacttagtc ccgagagcct agatattaaa gagatcaatg ggaataaaat cacctgccgg
721 ggtctggtgg agtacttcaa ggcttatata aagatctatc aaggtgaaga attaccacat
781 cccaaatcca tgttacaggc cacagcagaa gctaacaatt tagcagccgt ggcaactgcc
841 aaggacacat acaacaaaaa aatggaagag atttgtggtg gtgacaaacc atttctggcc
901 ccaaatggct tgcagaccaa acacctgcaa cttaaggaag aatctgtgaa gctattccga
961 ggggtgaaga agatgggtgg ggaagaattt agccggcgtt acctgcagca gttggagagt
1021 gaaatagatg aactttacat ccaatatatc aagcacaatg atagcaaaaa tatcttccat
1081 gcagctcgta ccccagccac actgtttgta gtcatcttta tcacatatgt gattgctggt
1141 gtgactggat tcattggttt ggacatcata gctagcctat gcaatatgat aatgggactg
1201 acccttatca ccctgtgcac ttgggcatat atccggtact ctggagaata ccgagagctg
1261 ggagctgtaa tagaccaggt ggctgcagct ctgtgggacc agggaagtac aaatgaggct
1321 ttgtacaagc tttacagtgc agcagcaacc cacagacatc tgtatcatca agctttccct
1381 acaccaaagt cggaatctac tgaacaatca gaaaagaaaa aaatttaa
//