LOCUS CR457141 786 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834A028D for
gene MASA, E-1 enzyme; complete cds, incl. stopcodon.
ACCESSION CR457141
VERSION CR457141.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 786)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 786)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834A028D, ORFNo 1918
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A028D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_021204 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..786
/db_xref="H-InvDB:HIT000267991"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834A028D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..786
/codon_start=1
/gene="MASA"
/db_xref="GOA:Q9UHY7"
/db_xref="H-InvDB:HIT000267991.11"
/db_xref="HGNC:HGNC:24599"
/db_xref="InterPro:IPR006439"
/db_xref="InterPro:IPR023214"
/db_xref="InterPro:IPR023943"
/db_xref="InterPro:IPR027511"
/db_xref="InterPro:IPR036412"
/db_xref="PDB:1YNS"
/db_xref="PDB:1ZS9"
/db_xref="UniProtKB/Swiss-Prot:Q9UHY7"
/protein_id="CAG33422.1"
/translation="MVVLSVPAEVTVILLDIEGTTTPIAFVKDILFPYIEENVKEYLQ
THWEEEECQQDVSLLRKQAEEDAHLDGAVPIPAASGNGVDDLQQMIQAVVDNVCWQMS
LDRKTTALKQLQGHMWRAAFTAGRMKAEFFADVVPAVRKWREAGMKVYIYSSGSVEAQ
KLLFGHSTEGDILELVDGHFDTKIGHKVESESYRKIADSIGCSTNNILFLTDVTREAS
AAEEADVHVAVVVRPGNAGLTDDEKTYYSLITSFSELYLPSST"
BASE COUNT 215 a 165 c 221 g 185 t
ORIGIN
1 atggtcgtgc tttcggtccc cgccgaagtc accgtgatcc tgttagatat cgaaggtacc
61 acaaccccga ttgctttcgt gaaggacatt ttatttcctt acatcgaaga aaatgttaaa
121 gagtatctgc agacacattg ggaagaagag gagtgccagc aggatgtcag tcttttgagg
181 aaacaggctg aagaggacgc ccacctggat ggggctgttc ctatccctgc agcatctggg
241 aatggagtgg atgatctgca acagatgatc caggccgtgg tagataatgt gtgctggcag
301 atgtccctgg atcgaaagac cactgcactc aaacagctgc agggccacat gtggagggcg
361 gcattcacag ctgggcgcat gaaagcagag ttctttgcag atgtagttcc agcagtcagg
421 aagtggagag aggccggaat gaaggtgtac atctattcct cagggagtgt ggaggcacag
481 aaactgttat tcgggcattc tacggaggga gatattcttg agcttgttga tggtcacttt
541 gataccaaga ttggacacaa agtagagagt gaaagttacc gaaagattgc agacagcatt
601 gggtgctcaa ccaacaacat tttgtttctg acagatgtta ctcgagaggc cagtgctgct
661 gaggaagcag atgtgcacgt agctgtggtg gtgagaccag gcaacgcagg attaacagat
721 gatgagaaga cttactacag cctcatcaca tccttcagtg aactatacct gccttcctca
781 acttaa
//