LOCUS CR457135 609 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834G127D for
gene PTTG1, pituitary tumor-transforming 1; complete cds, incl.
stopcodon.
ACCESSION CR457135
VERSION CR457135.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 609)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 609)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834G127D, ORFNo 1907
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834G127D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_004219 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..609
/db_xref="H-InvDB:HIT000267985"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834G127D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..609
/codon_start=1
/gene="PTTG1"
/db_xref="GOA:Q6IAL9"
/db_xref="H-InvDB:HIT000267985.12"
/db_xref="InterPro:IPR006940"
/db_xref="UniProtKB/TrEMBL:Q6IAL9"
/protein_id="CAG33416.1"
/translation="MATLIYVDKENGEPGTRVVAKDGLKLGSGPSIKALDGRSQVSTP
RFGKTFDAPPALPKATRKALGTVNRATEKSVKTKGPLKQKQPSFSAKKMTEKTVKAKS
SVPASDDAYPEIEKFFPFNPLDFESFDLPEEHQIAHLPLSGVPLMILDEERELEKLFQ
LGPPSPVKMPSPPWESNLLQSPSSILSTLDVELPPVCCDIDI"
BASE COUNT 171 a 149 c 136 g 153 t
ORIGIN
1 atggctactc tgatctatgt tgataaggaa aatggagaac caggcacccg tgtggttgct
61 aaggatgggc tgaagctggg gtctggacct tcaatcaaag ccttagatgg gagatctcaa
121 gtttcaacac cacgttttgg caaaacgttc gatgccccac cagccttacc taaagctact
181 agaaaggctt tgggaactgt caacagagct acagaaaagt ctgtaaagac caagggaccc
241 ctcaaacaaa aacagccaag cttttctgcc aaaaagatga ctgagaagac tgttaaagca
301 aaaagctctg ttcctgcctc agatgatgcc tatccagaaa tagaaaaatt ctttcccttc
361 aatcctctag actttgagag ttttgacctg cctgaagagc accagattgc gcacctcccc
421 ttgagtggag tgcctctcat gatccttgac gaggagagag agcttgaaaa gctgtttcag
481 ctgggccccc cttcacctgt gaagatgccc tctccaccat gggaatccaa tctgttgcag
541 tctccttcaa gcattctgtc gaccttggat gttgaattgc cacctgtttg ctgtgacata
601 gatatttaa
//