LOCUS CR457122 540 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834H087D for gene FAIM, Fas apoptotic inhibitory molecule; complete cds, incl. stopcodon. ACCESSION CR457122 VERSION CR457122.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 540) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 540) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834H087D, ORFNo 1873 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834H087D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is a part of a collection of human full length expression clones generated by RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC (ATG). The last base of the last coding triplett has been changed to T, which might lead to an amino acid change at the C terminus of the polypeptide. The stop codon has been set to TAA followed by TTAACCCAGCTTTCTT..att. Compared to the reference sequence NM_018147 we found amino acid exchange(s) at position (first base of changed triplet): 196(asn->ser) 349(ala->thr) Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..540 /db_xref="H-InvDB:HIT000267972" /organism="Homo sapiens" /lab_host="DH10B" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834H087D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..540 /codon_start=1 /gene="FAIM" /db_xref="GOA:Q9NVQ4" /db_xref="H-InvDB:HIT000267972.14" /db_xref="HGNC:HGNC:18703" /db_xref="InterPro:IPR010695" /db_xref="InterPro:IPR038513" /db_xref="PDB:2KW1" /db_xref="PDB:3MX7" /db_xref="UniProtKB/Swiss-Prot:Q9NVQ4" /protein_id="CAG33403.1" /translation="MTDLVAVWDVALSDGVHKIEFEHGTTSGKRVVYVDGKEEIRKEW MFKLVGKETFYVGAAKTKATISIDAISGFAYEYTLEINGKSLKKYMEDRSKTTNTWVL HMDGENFRIVLEKDTMDVWCNGKKLETAGEFVDDGTETHFSIGNHDCYIKAVSSGKRK EGIIHTLIVDNREIPEIAS" BASE COUNT 191 a 70 c 142 g 137 t ORIGIN 1 atgacagatc tcgtagctgt ttgggatgtt gctttaagtg acggagtcca caagatcgaa 61 tttgaacatg ggactacatc aggcaaacga gtagtatatg tagatggaaa ggaagagata 121 agaaaagagt ggatgttcaa attagtgggc aaagaaacat tctatgttgg agctgcaaag 181 acaaaagcga ccataagtat agacgctatc agtggttttg cttatgaata tactctggaa 241 attaatggga aaagtctcaa gaagtatatg gaggacagat caaaaaccac caatacttgg 301 gtattacaca tggatggtga gaactttaga attgttttgg aaaaagatac tatggacgta 361 tggtgcaatg gtaaaaaatt ggagacagcg ggtgagtttg tagatgatgg gactgaaact 421 cacttcagta tcgggaacca tgactgttac ataaaggctg tcagtagtgg gaagcggaaa 481 gaagggatta ttcatactct cattgtggat aatagagaaa tcccagagat tgcaagttaa //