LOCUS CR457120 762 bp mRNA linear HUM 03-JUN-2004
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834A109D for
gene FLJ10199, hypothetical protein FLJ10199; complete cds, incl.
stopcodon.
ACCESSION CR457120
VERSION CR457120.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 762)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 762)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834A109D, ORFNo 1869
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A109D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_018022 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..762
/db_xref="H-InvDB:HIT000267970"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834A109D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..762
/codon_start=1
/gene="FLJ10199"
/db_xref="GOA:Q9NW97"
/db_xref="H-InvDB:HIT000267970.12"
/db_xref="HGNC:HGNC:25488"
/db_xref="InterPro:IPR029265"
/db_xref="UniProtKB/Swiss-Prot:Q9NW97"
/protein_id="CAG33401.1"
/translation="MMAQSKANGSHYALTAIGLGMLVLGVIMAMWNLVPGFSAAEKPT
AQGSNKTEVGGGILKSKTFSVAYVLVGAGVMLLLLSICLSIRDKRKQRQGEDLAHVQH
PTGAGPHAQEEDSQEEEEEDEEAASRYYVPSYEEVMNTNYSEARGEEQNPRLSISLPS
YESLTGLDETTPTSTRADVEASPGNPPDRQNSKLAKRLKPLKVRRIKSEKLHLKDFRI
NLPDKNVPPPSIEPLTPPPQYDEVQEKAPDTRPPD"
BASE COUNT 189 a 223 c 226 g 124 t
ORIGIN
1 atgatggccc agtccaaggc caatggctcg cactatgcgc tgaccgccat cggcctgggg
61 atgctggtcc ttggggtgat catggccatg tggaacctgg tacccggctt cagcgcggcc
121 gagaagccaa cagctcaggg cagcaacaag accgaggtgg gtggcggcat cctcaagagc
181 aagaccttct ctgtggccta cgtgctggtc ggggccgggg tgatgctgct actgctttct
241 atctgcctga gtatcaggga taagaggaag cagcggcagg gcgaggacct ggcccatgtc
301 cagcacccga caggcgctgg gcctcacgcc caggaggaag acagccagga ggaagaagag
361 gaggatgagg aggctgcctc aaggtactat gttcccagct acgaggaagt gatgaacaca
421 aactactcag aagcaagggg agaggagcag aacccgaggt tgagcatctc tctcccgtcc
481 tatgagtcac tgacggggct cgacgagacc acccccacat ccaccagggc tgacgtggag
541 gccagccctg ggaacccccc tgacaggcag aactctaagt tggccaaacg actgaaaccg
601 ctgaaagttc gaaggattaa atctgaaaag cttcacctca aagactttag gatcaacctc
661 ccagacaaaa acgtccctcc tccctcgata gagcctttga ctcctccacc gcagtatgat
721 gaggtccagg agaaggcccc cgacacccgg ccgcccgatt aa
//