LOCUS CR457116 1176 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834F1012D for
gene HERPUD1, homocysteine-inducible, endoplasmic reticulum
stress-inducible, ubiquitin-like domain member 1; complete cds,
incl. stopcodon.
ACCESSION CR457116
VERSION CR457116.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1176)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1176)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834F1012D, ORFNo 1851
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834F1012D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_014685 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1176
/db_xref="H-InvDB:HIT000267966"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834F1012D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1176
/codon_start=1
/gene="HERPUD1"
/db_xref="GOA:Q15011"
/db_xref="H-InvDB:HIT000267966.12"
/db_xref="HGNC:HGNC:13744"
/db_xref="InterPro:IPR000626"
/db_xref="InterPro:IPR029071"
/db_xref="InterPro:IPR039751"
/db_xref="PDB:1WGD"
/db_xref="UniProtKB/Swiss-Prot:Q15011"
/protein_id="CAG33397.1"
/translation="MESETEPEPVTLLVKSPNQRHRDLELSGDRGWSVGHLKAHLSRV
YPERPRPEDQRLIYSGKLLLDHQCLRDLLPKQEKRHVLHLVCNVKSPSKMPEINAKVA
ESTEEPAGSNRGQYPEDSSSDGLRQREVLRNLSSPGWENISRPEAAQQAFQGLGPGFS
GYTPYGWLQLSWFQQIYARQYYMQYLAATAASGAFVPPPSAQEIPVVSAPAPAPIHNQ
FPAENQPANQNAAPQVVVNPGANQNLRMNAQGGPIVEEDDEINRDWLDWTYSAATFSV
FLSILYFYSSLSRFLMVMGATVVMYLHHVGWFPFRPRPVQNFPNDGPPPDVVNQDPNN
NLQEGTDPETEDPNHLPPDRDVLDGEQTSPSFMSTAWLVFKTFFASLLPEGPPAIAN"
BASE COUNT 284 a 326 c 283 g 283 t
ORIGIN
1 atggagtccg agaccgaacc cgagcccgtc acgctcctgg tgaagagccc caaccagcgc
61 caccgcgact tggagctgag tggcgaccgc ggctggagtg tgggccacct caaggcccac
121 ctgagccgcg tctaccccga gcgtccgcgt ccagaggacc agaggttaat ttattctggg
181 aagctgttgt tggatcacca atgtctcagg gacttgcttc caaagcagga aaaacggcat
241 gttttgcatc tggtgtgcaa tgtgaagagt ccttcaaaaa tgccagaaat caacgccaag
301 gtggctgaat ccacagagga gcctgctggt tctaatcggg gacagtatcc tgaggattcc
361 tcaagtgatg gtttaaggca aagggaagtt cttcggaacc tttcttcccc tggatgggaa
421 aacatctcaa ggcctgaagc tgcccagcag gcattccaag gcctgggtcc tggtttctcc
481 ggttacacac cctatgggtg gcttcagctt tcctggttcc agcagatata tgcacgacag
541 tactacatgc aatatttagc agccactgct gcatcagggg cttttgttcc accaccaagt
601 gcacaagaga tacctgtggt ctctgcacct gctccagccc ctattcacaa ccagtttcca
661 gctgaaaacc agcctgccaa tcagaatgct gctcctcaag tggttgttaa tcctggagcc
721 aatcaaaatt tgcggatgaa tgcacaaggt ggccctattg tggaagaaga tgatgaaata
781 aatcgagatt ggttggattg gacctattca gcagctacat tttctgtttt tctcagtatc
841 ctctacttct actcctccct gagcagattc ctcatggtca tgggggccac cgttgttatg
901 tacctgcatc acgttgggtg gtttccattt agaccgaggc cggttcagaa cttcccaaat
961 gatggtcctc ctcctgacgt tgtaaatcag gaccccaaca ataacttaca ggaaggcact
1021 gatcctgaaa ctgaagaccc caaccacctc cctccagaca gggatgtact agatggcgag
1081 cagaccagcc cctcctttat gagcacagca tggcttgtct tcaagacttt ctttgcctct
1141 cttcttccag aaggcccccc agccatcgca aattaa
//