LOCUS CR457102 510 bp mRNA linear HUM 03-JUN-2004
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834F067D for
gene SDHC, succinate dehydrogenase complex, subunit C, integral
membrane protein, 15kDa; complete cds, incl. stopcodon.
ACCESSION CR457102
VERSION CR457102.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 510)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 510)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834F067D, ORFNo 1821
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834F067D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_003001 we found amino acid
exchange(s) at position (first base of changed triplet):
7(ala->val) 490(met->ile) 505(met->ile)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..510
/db_xref="H-InvDB:HIT000267952"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834F067D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..510
/codon_start=1
/gene="SDHC"
/db_xref="GOA:Q6IAQ2"
/db_xref="H-InvDB:HIT000267952.13"
/db_xref="InterPro:IPR000701"
/db_xref="InterPro:IPR014314"
/db_xref="InterPro:IPR018495"
/db_xref="InterPro:IPR034804"
/db_xref="UniProtKB/TrEMBL:Q6IAQ2"
/protein_id="CAG33383.1"
/translation="MAVLLLRHVGRHCLRAHFSPQLCIRNAVPLGTTAKEEMERFWNK
NIGSNRPLSPHITIYSWSLPMAMSICHRGTGIALSAGVSLFGMSALLLPGNFESYLEL
VKSLCLGPALIHTAKFALVFPLMYHTWNGIRHLMWDLGKGLKIPQLYQSGVVVLVLTV
LSSIGLAAI"
BASE COUNT 100 a 130 c 127 g 153 t
ORIGIN
1 atggctgtgc tgttgctgag acacgttggt cgtcattgcc tccgagccca ctttagccct
61 cagctctgta tcagaaatgc tgttcctttg ggaaccacgg ccaaagaaga gatggagcgg
121 ttctggaata agaatatagg ttcaaaccgt cctctgtctc cccacattac tatctacagt
181 tggtctcttc ccatggcgat gtccatctgc caccgtggca ctggtattgc tttgagtgca
241 ggggtctctc tttttggcat gtcggccctg ttactccctg ggaactttga gtcttatttg
301 gaacttgtga agtccctgtg tctggggcca gcactgatcc acacagctaa gtttgcactt
361 gtcttccctc tcatgtatca tacctggaat gggatccgac acttgatgtg ggacctagga
421 aaaggcctga agattcccca gctataccag tctggagtgg ttgtcctggt tcttactgtg
481 ttgtcctcta tagggctggc agccatttaa
//