LOCUS CR457098 1005 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B0419D for
gene B3GAT1, beta-1,3-glucuronyltransferase 1
(glucuronosyltransferase P); complete cds, incl. stopcodon.
ACCESSION CR457098
VERSION CR457098.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1005)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1005)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B0419D, ORFNo 1811
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B0419D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_054025 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1005
/db_xref="H-InvDB:HIT000267948"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B0419D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1005
/codon_start=1
/gene="B3GAT1"
/db_xref="GOA:Q9P2W7"
/db_xref="H-InvDB:HIT000267948.12"
/db_xref="HGNC:HGNC:921"
/db_xref="InterPro:IPR005027"
/db_xref="InterPro:IPR029044"
/db_xref="PDB:1V82"
/db_xref="PDB:1V83"
/db_xref="PDB:1V84"
/db_xref="UniProtKB/Swiss-Prot:Q9P2W7"
/protein_id="CAG33379.1"
/translation="MPKRRDILAIVLIVLPWTLLITVWHQSTLAPLLAVHKDEGSDPR
RETPPGADPREYCTSDRDIVEVVRTEYVYTRPPPWSDTLPTIHVVTPTYSRPVQKAEL
TRMANTLLHVPNLHWLVVEDAPRRTPLTARLLRDTGLNYTHLHVETPRNYKLRGDARD
PRIPRGTMQRNLALRWLRETFPRNSSQPGVVYFADDDNTYSLELFEEMRSTRRVSVWP
VAFVGGLRYEAPRVNGAGKVVGWKTVFDPHRPFAIDMAGFAVNLRLILQRSQAYFKLR
GVKGGYQESSLLRELVTLNDLEPKAANCTKILVWHTRTEKPVLVNEGKKGFTDPSVEI
"
BASE COUNT 199 a 346 c 310 g 150 t
ORIGIN
1 atgccgaaga gacgggacat cctagcgatc gtcctcatcg tgctgccctg gactctgctc
61 atcactgtct ggcaccagag caccctcgca cccctgctcg cggtacataa ggatgagggc
121 agtgaccccc gacgcgaaac gccgcccggc gccgacccca gggagtactg cacgtctgac
181 cgcgacatcg tggaggtggt gcgcaccgag tacgtgtaca cgcggccccc gccatggtcc
241 gacacgctgc ccaccatcca cgtggtgacg cccacctaca gccgcccggt gcagaaggcc
301 gagctgacgc gcatggccaa cacgctgctg cacgtgccca acctccactg gctggtggtg
361 gaggatgcgc cgcgccggac gccgctgacc gcgcgcctgc tgcgcgacac cggcctcaac
421 tacacgcacc tgcacgtgga gacgccccgc aactacaagc tgcgcggaga cgcccgcgac
481 ccacgcatcc cgcggggcac catgcagcgc aacctggccc tgcgctggct gcgcgagacc
541 ttcccgcgca actccagcca gcctggcgtg gtctacttcg ccgacgacga caacacctac
601 agcctggagc tcttcgaaga gatgcgcagc accaggaggg tgtccgtgtg gcccgtcgcc
661 ttcgtgggtg gcctgcggta cgaggcccca cgggtgaacg gggcagggaa ggtggtcggc
721 tggaagacgg tgtttgaccc ccaccggcca tttgcaatag acatggctgg atttgccgtc
781 aacctgcggc tcattctgca gcgaagccag gcctacttca agctgcgagg tgtgaaggga
841 ggctaccagg aaagcagcct ccttcgagaa cttgtcaccc tcaacgacct ggagcccaag
901 gcagccaact gcaccaagat cctggtgtgg cacacacgga cagagaagcc agtgctggtg
961 aatgagggca agaagggctt cactgacccc tcggtggaga tttaa
//