LOCUS CR457094 597 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834F077D for
gene DUSP13, dual specificity phosphatase 13; complete cds, incl.
stopcodon.
ACCESSION CR457094
VERSION CR457094.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 597)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 597)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834F077D, ORFNo 1806
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834F077D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_016364 we found amino acid
exchange(s) at position (first base of changed triplet):
244(lys->glu)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..597
/db_xref="H-InvDB:HIT000267944"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834F077D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..597
/codon_start=1
/gene="DUSP13"
/db_xref="GOA:Q9UII6"
/db_xref="H-InvDB:HIT000267944.12"
/db_xref="HGNC:HGNC:19681"
/db_xref="InterPro:IPR000340"
/db_xref="InterPro:IPR000387"
/db_xref="InterPro:IPR016130"
/db_xref="InterPro:IPR020405"
/db_xref="InterPro:IPR020417"
/db_xref="InterPro:IPR020422"
/db_xref="InterPro:IPR029021"
/db_xref="PDB:2GWO"
/db_xref="PDB:2PQ5"
/db_xref="UniProtKB/Swiss-Prot:Q9UII6"
/protein_id="CAG33375.1"
/translation="MDSLQKQDLRRPKIHGAVQASPYQPPTLASLQRLLWVRQAATLN
HIDEVWPSLFLGDAYAARDKSKLIQLGITHVVNAAAGEFQVDTGAKFYRGMSLEYYGI
EADDNPFFDLSVYFLPVARYIRAALSVPQGRVLVHCAMGVSRSATLVLAFLMIYENMT
LVEAIQTVQAHRNICPNSGFLRQLQVLDNRLGRETGRF"
BASE COUNT 118 a 186 c 169 g 124 t
ORIGIN
1 atggactcac tgcagaagca ggacctccgg aggcccaaga tccatggggc agtccaggca
61 tctccctacc agccgcccac attggcttcg ctgcagcgct tgctgtgggt ccgtcaggct
121 gccacactga accatatcga tgaggtctgg cccagcctct tcctgggaga tgcgtacgca
181 gcccgggaca agagcaagct gatccagctg ggaatcaccc acgttgtgaa tgccgctgca
241 ggcgagttcc aggtggacac aggtgccaaa ttctaccgtg gaatgtccct ggagtactat
301 ggcattgagg cggacgacaa ccccttcttc gacctcagtg tctactttct gcctgttgct
361 cgatacatcc gagctgccct cagtgttccc caaggccgcg tgctggtaca ctgtgccatg
421 ggggtaagcc gctctgccac acttgtcctg gccttcctca tgatctatga gaacatgacg
481 ctggtagagg ccatccagac ggtgcaggcc caccgcaata tctgccctaa ctcaggcttc
541 ctccggcagc tccaggttct ggacaaccga ctggggcggg agacggggcg gttttaa
//