LOCUS       CR457076                 666 bp    mRNA    linear   HUM 16-OCT-2008
DEFINITION  Homo sapiens full open reading frame cDNA clone RZPDo834D107D for
            gene RAB27A, RAB27A, member RAS oncogene family; complete cds,
            incl. stopcodon.
ACCESSION   CR457076
VERSION     CR457076.1
KEYWORDS    Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
  TITLE     Cloning of human full open reading frames in Gateway(TM) system
            entry vector (pDONR201)
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 666)
  AUTHORS   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
  JOURNAL   Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
            Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
            580, D-69120 Heidelberg, Germany
COMMENT     RZPD; RZPDo834D107D, ORFNo 1754
            www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D107D
            RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
            RZPD LIB No. 834
            www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
            www.rzpd.de/products/orfclones/
            Contact: Inge Arlart
            RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
            Heubnerweg 6, D-14059 Berlin, Germany
            Tel: +49 30 32639 100
            Fax: +49 30 32639 111
            www.rzpd.de
            This clone is available from RZPD;
            contact RZPD (customer.service@rzpd.de) for further information.
            
            This CDS clone is a part of a collection of human full length
            expression clones generated by RZPD.
            This CDS has been cloned incl. stopcodon.
            The CDS has been inserted into pDONR201 via a BP Clonase(TM)
            reaction.
            Additional sequence has been added in front of the start codon:
            att..AAAAAA GCA GGC (ATG).
            The last base of the last coding triplett has been changed to T,
            which might lead to an amino acid change at the C terminus of the
            polypeptide.
            The stop codon has been set to TAA followed by
            TTAACCCAGCTTTCTT..att.
            Compared to the reference sequence NM_183235 we found amino acid
            exchange(s) at position (first base of changed triplet):
            259(ala->thr)
            Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES             Location/Qualifiers
     source          1..666
                     /db_xref="H-InvDB:HIT000267926"
                     /organism="Homo sapiens"
                     /lab_host="DH10B"
                     /mol_type="mRNA"
                     /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
                     /clone="RZPDo834D107D"
                     /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
                     /db_xref="taxon:9606"
     CDS             1..666
                     /codon_start=1
                     /gene="RAB27A"
                     /db_xref="GOA:Q6IAS8"
                     /db_xref="H-InvDB:HIT000267926.12"
                     /db_xref="InterPro:IPR001806"
                     /db_xref="InterPro:IPR005225"
                     /db_xref="InterPro:IPR027417"
                     /db_xref="InterPro:IPR041837"
                     /db_xref="UniProtKB/TrEMBL:Q6IAS8"
                     /protein_id="CAG33357.1"
                     /translation="MSDGDYDYLIKFLALGDSGVGKTSVLYQYTDGKFNSKFITTVGI
                     DFREKRVVYRASGPDGATGRGQRIHLQLWDTAGQERFRSLTTTFFRDAMGFLLLFDLT
                     NEQSFLNVRNWISQLQMHAYCENPDIVLCGNKSDLEDQRVVKEEEAIALAEKYGIPYF
                     ETSAANGTNISQAIEMLLDLIMKRMERCVDKSWIPEGVVRSNGHASTDQLSEEKEKGA
                     CGC"
BASE COUNT          207 a          116 c          183 g          160 t
ORIGIN      
        1 atgtctgatg gagattatga ttacctcatc aagtttttag ctttgggaga ctctggtgta
       61 gggaagacca gtgtacttta ccaatataca gatggtaaat ttaactccaa atttatcaca
      121 acagtgggca ttgatttcag ggaaaaaaga gtggtgtaca gagccagtgg gccggatgga
      181 gccactggca gaggccagag aatccacctg cagttatggg acacagcagg gcaggagagg
      241 tttcgtagct taacgacaac gttcttcaga gatgctatgg gttttcttct actttttgat
      301 ctgacaaatg agcaaagttt cctcaatgtc agaaactgga taagccagct acagatgcat
      361 gcatattgtg aaaacccaga tatagtgctg tgtggaaaca agagtgatct ggaggaccag
      421 agagtagtga aagaggagga agccatagca ctcgcagaga aatatggaat cccctacttt
      481 gaaactagtg ctgccaatgg gacaaacata agccaagcaa ttgagatgct tctggacctg
      541 ataatgaagc gaatggaacg gtgtgtggac aagtcctgga ttcctgaagg agtggtgcga
      601 tcaaatggtc atgcctctac ggatcagtta agtgaagaaa aggagaaagg ggcatgtggc
      661 tgttaa
//