LOCUS CR457076 666 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D107D for gene RAB27A, RAB27A, member RAS oncogene family; complete cds, incl. stopcodon. ACCESSION CR457076 VERSION CR457076.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 666) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 666) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834D107D, ORFNo 1754 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D107D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is a part of a collection of human full length expression clones generated by RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC (ATG). The last base of the last coding triplett has been changed to T, which might lead to an amino acid change at the C terminus of the polypeptide. The stop codon has been set to TAA followed by TTAACCCAGCTTTCTT..att. Compared to the reference sequence NM_183235 we found amino acid exchange(s) at position (first base of changed triplet): 259(ala->thr) Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..666 /db_xref="H-InvDB:HIT000267926" /organism="Homo sapiens" /lab_host="DH10B" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834D107D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..666 /codon_start=1 /gene="RAB27A" /db_xref="GOA:Q6IAS8" /db_xref="H-InvDB:HIT000267926.12" /db_xref="InterPro:IPR001806" /db_xref="InterPro:IPR005225" /db_xref="InterPro:IPR027417" /db_xref="InterPro:IPR041837" /db_xref="UniProtKB/TrEMBL:Q6IAS8" /protein_id="CAG33357.1" /translation="MSDGDYDYLIKFLALGDSGVGKTSVLYQYTDGKFNSKFITTVGI DFREKRVVYRASGPDGATGRGQRIHLQLWDTAGQERFRSLTTTFFRDAMGFLLLFDLT NEQSFLNVRNWISQLQMHAYCENPDIVLCGNKSDLEDQRVVKEEEAIALAEKYGIPYF ETSAANGTNISQAIEMLLDLIMKRMERCVDKSWIPEGVVRSNGHASTDQLSEEKEKGA CGC" BASE COUNT 207 a 116 c 183 g 160 t ORIGIN 1 atgtctgatg gagattatga ttacctcatc aagtttttag ctttgggaga ctctggtgta 61 gggaagacca gtgtacttta ccaatataca gatggtaaat ttaactccaa atttatcaca 121 acagtgggca ttgatttcag ggaaaaaaga gtggtgtaca gagccagtgg gccggatgga 181 gccactggca gaggccagag aatccacctg cagttatggg acacagcagg gcaggagagg 241 tttcgtagct taacgacaac gttcttcaga gatgctatgg gttttcttct actttttgat 301 ctgacaaatg agcaaagttt cctcaatgtc agaaactgga taagccagct acagatgcat 361 gcatattgtg aaaacccaga tatagtgctg tgtggaaaca agagtgatct ggaggaccag 421 agagtagtga aagaggagga agccatagca ctcgcagaga aatatggaat cccctacttt 481 gaaactagtg ctgccaatgg gacaaacata agccaagcaa ttgagatgct tctggacctg 541 ataatgaagc gaatggaacg gtgtgtggac aagtcctgga ttcctgaagg agtggtgcga 601 tcaaatggtc atgcctctac ggatcagtta agtgaagaaa aggagaaagg ggcatgtggc 661 tgttaa //