LOCUS CR457054 1428 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834C0418D for
gene LPL, lipoprotein lipase; complete cds, incl. stopcodon.
ACCESSION CR457054
VERSION CR457054.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1428)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1428)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834C0418D, ORFNo 1663
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834C0418D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_000237 we found amino acid
exchange(s) at position (first base of changed triplet):
277(pro->ser) 811(ser->phe)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1428
/db_xref="H-InvDB:HIT000267904"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834C0418D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1428
/codon_start=1
/gene="LPL"
/db_xref="GOA:Q6IAV0"
/db_xref="H-InvDB:HIT000267904.13"
/db_xref="InterPro:IPR000734"
/db_xref="InterPro:IPR001024"
/db_xref="InterPro:IPR002330"
/db_xref="InterPro:IPR013818"
/db_xref="InterPro:IPR016272"
/db_xref="InterPro:IPR029058"
/db_xref="InterPro:IPR033906"
/db_xref="InterPro:IPR036392"
/db_xref="UniProtKB/TrEMBL:Q6IAV0"
/protein_id="CAG33335.1"
/translation="MESKALLVLTLAVWLQSLTASRGGVAAADQRRDFIDIESKFALR
TPEDTAEDTCHLIPGVAESVATCHFNHSSKTFMVIHGWTVTGMYESWVSKLVAALYKR
EPDSNVIVVDWLSRAQEHYPVSAGYTKLVGQDVARFINWMEEEFNYPLDNVHLLGYSL
GAHAAGIAGSLTNKKVNRITGLDPAGPNFEYAEAPSRLSPDDADFVDVLHTFTRGSPG
RSIGIQKPVGHVDIYPNGGTFQPGCNIGEAIRVIAERGLGDVDQLVKCSHERFIHLFI
DSLLNEENPSKAYRCSSKEAFEKGLCLSCRKNRCNNLGYEINKVRAKRSSKMYLKTRS
QMPYKVFHYQVKIHFSGTESETHTNQAFEISLYGTVAESENIPFTLPEVSTNKTYSFL
IYTEVDIGELLMLKLKWKSDSYFSWSDWWSSPGFAIQKIRVKAGETQKKVIFCSREKV
SHLQKGKAPAVFVKCHDKSLNKKSG"
BASE COUNT 397 a 330 c 366 g 335 t
ORIGIN
1 atggagagca aagccctgct cgtgctgact ctggccgtgt ggctccagag tctgaccgcc
61 tcccgcggag gggtggccgc cgccgaccaa agaagagatt ttatcgacat cgaaagtaaa
121 tttgccctaa ggacccctga agacacagct gaggacactt gccacctcat tcccggagta
181 gcagagtccg tggctacctg tcatttcaat cacagcagca aaaccttcat ggtgatccat
241 ggctggacgg taacaggaat gtatgagagt tgggtgtcaa aacttgtggc cgccctgtac
301 aagagagaac cagactccaa tgtcattgtg gtggactggc tgtcacgggc tcaggagcat
361 tacccagtgt ccgcgggcta caccaaactg gtgggacagg atgtggcccg gtttatcaac
421 tggatggagg aggagtttaa ctaccctctg gacaatgtcc atctcttggg atacagcctt
481 ggagcccatg ctgctggcat tgcaggaagt ctgaccaata agaaagtcaa cagaattact
541 ggcctcgatc cagctggacc taactttgag tatgcagaag ccccgagtcg tctttctcct
601 gatgatgcag attttgtaga cgtcttacac acattcacca gagggtcccc tggtcgaagc
661 attggaatcc agaaaccagt tgggcatgtt gacatttacc cgaatggagg tacttttcag
721 ccaggatgta acattggaga agctatccgc gtgattgcag agagaggact tggagatgtg
781 gaccagctag tgaagtgctc ccacgagcgc ttcattcatc tcttcatcga ctctctgttg
841 aatgaagaaa atccaagtaa ggcctacagg tgcagttcca aggaagcctt tgagaaaggg
901 ctctgcttga gttgtagaaa gaaccgctgc aacaatctgg gctatgagat caataaagtc
961 agagccaaaa gaagcagcaa aatgtacctg aagactcgtt ctcagatgcc ctacaaagtc
1021 ttccattacc aagtaaagat tcatttttct gggactgaga gtgaaaccca taccaatcag
1081 gcctttgaga tttctctgta tggcaccgtg gccgagagtg agaacatccc attcactctg
1141 cctgaagttt ccacaaataa gacctactcc ttcctaattt acacagaggt agatattgga
1201 gaactactca tgttgaagct caaatggaag agtgattcat actttagctg gtcagactgg
1261 tggagcagtc ccggcttcgc cattcagaag atcagagtaa aagcaggaga gactcagaaa
1321 aaggtgatct tctgttctag ggagaaagtg tctcatttgc agaaaggaaa ggcacctgcg
1381 gtatttgtga aatgccatga caagtctctg aataagaagt caggttaa
//