LOCUS CR457053 1002 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B0418D for
gene CTSL, cathepsin L; complete cds, incl. stopcodon.
ACCESSION CR457053
VERSION CR457053.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1002)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1002)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B0418D, ORFNo 1661
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B0418D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_001912 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1002
/db_xref="H-InvDB:HIT000267903"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B0418D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1002
/codon_start=1
/gene="CTSL"
/db_xref="GOA:P07711"
/db_xref="H-InvDB:HIT000267903.13"
/db_xref="HGNC:HGNC:2537"
/db_xref="InterPro:IPR000169"
/db_xref="InterPro:IPR000668"
/db_xref="InterPro:IPR013201"
/db_xref="InterPro:IPR025660"
/db_xref="InterPro:IPR025661"
/db_xref="InterPro:IPR038765"
/db_xref="InterPro:IPR039417"
/db_xref="PDB:1CJL"
/db_xref="PDB:1CS8"
/db_xref="PDB:1ICF"
/db_xref="PDB:1MHW"
/db_xref="PDB:2NQD"
/db_xref="PDB:2VHS"
/db_xref="PDB:2XU1"
/db_xref="PDB:2XU3"
/db_xref="PDB:2XU4"
/db_xref="PDB:2XU5"
/db_xref="PDB:2YJ2"
/db_xref="PDB:2YJ8"
/db_xref="PDB:2YJ9"
/db_xref="PDB:2YJB"
/db_xref="PDB:2YJC"
/db_xref="PDB:3BC3"
/db_xref="PDB:3H89"
/db_xref="PDB:3H8B"
/db_xref="PDB:3H8C"
/db_xref="PDB:3HHA"
/db_xref="PDB:3HWN"
/db_xref="PDB:3IV2"
/db_xref="PDB:3K24"
/db_xref="PDB:3KSE"
/db_xref="PDB:3OF8"
/db_xref="PDB:3OF9"
/db_xref="PDB:4AXL"
/db_xref="PDB:4AXM"
/db_xref="PDB:5F02"
/db_xref="PDB:5I4H"
/db_xref="PDB:5MAE"
/db_xref="PDB:5MAJ"
/db_xref="PDB:5MQY"
/db_xref="PDB:6EZP"
/db_xref="PDB:6EZX"
/db_xref="PDB:6F06"
/db_xref="UniProtKB/Swiss-Prot:P07711"
/protein_id="CAG33334.1"
/translation="MNPTLILAAFCLGIASATLTFDHSLEAQWTKWKAMHNRLYGMNE
EGWRRAVWEKNMKMIELHNQEYREGKHSFTMAMNAFGDMTSEEFRQVMNGFQNRKPRK
GKVFQEPLFYEAPRSVDWREKGYVTPVKNQGQCGSCWAFSATGALEGQMFRKTGRLIS
LSEQNLVDCSGPQGNEGCNGGLMDYAFQYVQDNGGLDSEESYPYEATEESCKYNPKYS
VANDTGFVDIPKQEKALMKAVATVGPISVAIDAGHESFLFYKEGIYFEPDCSSEDMDH
GVLVVGYGFESTESDNNKYWLVKNSWGEEWGMGGYVKMAKDRRNHCGIASAASYPTV"
BASE COUNT 284 a 192 c 285 g 241 t
ORIGIN
1 atgaatccta cactcatcct tgctgccttt tgcctgggaa ttgcctcagc tactctaaca
61 tttgatcaca gtttagaggc acagtggacc aagtggaagg cgatgcacaa cagattatac
121 ggcatgaatg aagaaggatg gaggagagca gtgtgggaga agaacatgaa gatgattgaa
181 ctgcacaatc aggaatacag ggaagggaaa cacagcttca caatggccat gaacgccttt
241 ggagacatga ccagtgaaga attcaggcag gtgatgaatg gctttcaaaa ccgtaagccc
301 aggaagggga aagtgttcca ggaacctctg ttttatgagg cccccagatc tgtggattgg
361 agagagaaag gctacgtgac tcctgtgaag aatcagggtc agtgtggttc ttgttgggct
421 tttagtgcta ctggtgctct tgaaggacag atgttccgga aaactgggag gcttatctca
481 ctgagtgagc agaatctggt agactgctct gggcctcaag gcaatgaagg ctgcaatggt
541 ggcctaatgg attatgcttt ccagtatgtt caggataatg gaggcctgga ctctgaggaa
601 tcctatccat atgaggcaac agaagaatcc tgtaagtaca atcccaagta ttctgttgct
661 aatgacaccg gctttgtgga catccctaag caggagaagg ccctgatgaa ggcagttgca
721 actgtggggc ccatttctgt tgctattgat gcaggtcatg agtccttcct gttctataaa
781 gaaggcattt attttgagcc agactgtagc agtgaagaca tggatcatgg tgtgctggtg
841 gttggctacg gatttgaaag cacagaatca gataacaata aatattggct ggtgaagaac
901 agctggggtg aagaatgggg catgggtggc tacgtaaaga tggccaaaga ccggagaaac
961 cattgtggaa ttgcctcagc agccagctac cccactgttt aa
//