LOCUS CR457033 1254 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B0218D for
gene FDFT1, farnesyl-diphosphate farnesyltransferase 1; complete
cds, incl. stopcodon.
ACCESSION CR457033
VERSION CR457033.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1254)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1254)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B0218D, ORFNo 1606
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B0218D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence BC003573 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1254
/db_xref="H-InvDB:HIT000267883"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B0218D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1254
/codon_start=1
/gene="FDFT1"
/db_xref="GOA:Q6IAX1"
/db_xref="H-InvDB:HIT000267883.12"
/db_xref="InterPro:IPR002060"
/db_xref="InterPro:IPR006449"
/db_xref="InterPro:IPR008949"
/db_xref="InterPro:IPR019845"
/db_xref="InterPro:IPR033904"
/db_xref="UniProtKB/TrEMBL:Q6IAX1"
/protein_id="CAG33314.1"
/translation="MEFVKCLGHPEEFYNLVRFRIGGKRKVMPKMDQDSLSSSLKTCY
KYLNQTSRSFAAVIQALDGEMRNAVCIFYLVLRALDTLEDDMTISVEKKVPLLHNFHS
FLYQPDWRFMESKEKDRQVLEDFPTISLEFRNLAEKYQTVIADICRRMGIGMAEFLDK
HVTSEQEWDKYCHYVAGLVGIGLSRLFSASEFEDPLVGEDTERANSMGLFLQKTNIIR
DYLEDQQGGREFWPQEVWSRYVKKLGDFAKPENIDLAVQCLNELITNALHHIPDVITY
LSRLRNQSVFNFCAIPQVMAIATLAACYNNQQVFKGAVKIRKGQAVTLMMDATNMPAV
KAIIYQYMEEIYHRIPDSDPSSSKTRQIISTIRTQNLPNCQLISRSHYSPIYLSFVML
LAALSWQYLTTLSQVTEDYVQTGEH"
BASE COUNT 332 a 306 c 313 g 303 t
ORIGIN
1 atggagttcg tgaaatgcct tggccacccc gaagagttct acaacctggt gcgcttccgg
61 atcgggggca agcggaaggt gatgcccaag atggaccagg actcgctcag cagcagcctg
121 aaaacttgct acaagtatct caatcagacc agtcgcagtt tcgcagctgt tatccaggcg
181 ctggatgggg aaatgcgcaa tgcagtgtgc atattttatc tggttctccg agctctggac
241 acactggaag atgacatgac catcagtgtg gaaaagaagg tcccgctgtt acacaacttt
301 cactctttcc tttaccaacc agactggcgg ttcatggaga gcaaggagaa ggatcgccag
361 gtgctggagg acttcccaac gatctccctt gagtttagaa atctggctga gaaataccaa
421 acagtgattg ccgacatttg ccggagaatg ggcattggga tggcagagtt tttggataag
481 catgtgacct ctgaacagga gtgggacaag tactgccact atgttgctgg gctggtcgga
541 attggccttt cccgtctttt ctcagcctca gagtttgaag accccttagt tggtgaagat
601 acagaacgtg ccaactctat gggcctgttt ctgcagaaaa caaacatcat ccgtgactat
661 ctggaagacc agcaaggagg aagagagttc tggcctcaag aggtttggag caggtatgtt
721 aagaagttag gggattttgc taagccggag aatattgact tggccgtgca gtgcctgaat
781 gaacttataa ccaatgcact gcaccacatc ccagatgtca tcacctacct ttcgagactc
841 agaaaccaga gtgtgtttaa cttctgtgct attccacagg tgatggccat tgccactttg
901 gctgcctgtt ataataacca gcaggtgttc aaaggggcag tgaagattcg gaaagggcaa
961 gcagtgaccc tcatgatgga tgccaccaat atgccagctg tcaaagccat catatatcag
1021 tatatggaag agatttatca tagaatcccc gactcagacc catcttctag caaaacaagg
1081 cagatcatct ccaccatccg gacgcagaat cttcccaact gtcagctgat ttcccgaagc
1141 cactactccc ccatctacct gtcgtttgtc atgcttttgg ctgccctgag ctggcagtac
1201 ctgaccactc tctcccaggt aacagaagac tatgttcaga ctggagaaca ttaa
//