LOCUS CR457032 483 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D0315D for
gene RPL21, ribosomal protein L21; complete cds, incl. stopcodon.
ACCESSION CR457032
VERSION CR457032.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 483)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 483)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834D0315D, ORFNo 1605
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D0315D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_000982 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..483
/db_xref="H-InvDB:HIT000267882_04"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834D0315D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..483
/codon_start=1
/gene="RPL21"
/db_xref="GOA:Q6IAX2"
/db_xref="H-InvDB:HIT000267882_03.4"
/db_xref="InterPro:IPR001147"
/db_xref="InterPro:IPR008991"
/db_xref="InterPro:IPR018259"
/db_xref="InterPro:IPR036948"
/db_xref="UniProtKB/TrEMBL:Q6IAX2"
/protein_id="CAG33313.1"
/translation="MTNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKG
MGTVQKGMPHKCYHGKTGRVYNVTQHAVGIVVNKQVKGKILAKRINVRIEHIKHSKSR
DSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA
"
BASE COUNT 167 a 92 c 117 g 107 t
ORIGIN
1 atgacgaaca caaagggaaa gaggagaggc acccgatata tgttctctag gccttttaga
61 aaacatggag ttgttccttt ggccacatat atgcgaatct ataagaaagg tgatattgta
121 gacatcaagg gaatgggtac tgttcaaaaa ggaatgcccc acaagtgtta ccatggcaaa
181 actggaagag tctacaatgt tacccagcat gctgttggca ttgttgtaaa caaacaagtt
241 aagggcaaga ttcttgccaa gagaattaat gtgcgtattg agcacattaa gcactctaag
301 agccgagata gcttcctgaa acgtgtgaag gaaaatgatc agaaaaagaa agaagccaaa
361 gagaaaggta cctgggttca actaaagcgc cagcctgctc cacccagaga agcacacttt
421 gtgagaacca atgggaagga gcctgagctg ctggaaccta ttccctatga attcatggct
481 taa
//