LOCUS CR457029 1338 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834C0118D for
gene EIF3S6, eukaryotic translation initiation factor 3, subunit 6
48kDa; complete cds, incl. stopcodon.
ACCESSION CR457029
VERSION CR457029.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1338)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1338)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834C0118D, ORFNo 1596
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834C0118D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_001568 we found amino acid
exchange(s) at position (first base of changed triplet):
814(gln->leu)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1338
/db_xref="H-InvDB:HIT000267879"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834C0118D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1338
/codon_start=1
/gene="EIF3S6"
/db_xref="GOA:Q6IAX5"
/db_xref="H-InvDB:HIT000267879.15"
/db_xref="InterPro:IPR000717"
/db_xref="InterPro:IPR016650"
/db_xref="InterPro:IPR019010"
/db_xref="InterPro:IPR036388"
/db_xref="InterPro:IPR036390"
/db_xref="UniProtKB/TrEMBL:Q6IAX5"
/protein_id="CAG33310.1"
/translation="MAEYDLTTRIAHFLDRHLVFPLLEFLSVKEIYNEKELLQGKLDL
LSDTNMVDFAMDVYKNLYSDDIPHALREKRTTVVAQLKQLQAETEPIVKMFEDPETTR
QMQSTRDGRMLFDYLADKHGFRQEYLDTLYRYAKFQYECGNYSGAAEYLYFFRVLVPA
TDRNALSSLWGKLASEILMQNWDAAMEDLTRLKETIDNNSVSSPLQSLQQRTWLIHWS
LFVFFNHPKGRDNIIDLFLYQPQYLNAIQTMCPHILRYLTTAVITNKDVRKRRLVLKD
LVKVIQQESYTYKDPITEFVECLYVNFDFDGAQKKLRECESVLVNDFFLVACLEDFIE
NARLFIFETFCRIHQCISINMLADKLNMTPEEAERWIVNLIRNARLDAKIDSKLGHVV
MGNNAVSPYQQVIEKTKSLSFRSQMLAMNIEKKLNQNSRSEAPNWATQDSGFY"
BASE COUNT 416 a 249 c 281 g 392 t
ORIGIN
1 atggcggagt acgacttgac tactcgcatc gcgcactttt tggatcggca tctagtcttt
61 ccgcttcttg aatttctctc tgtaaaggag atatataatg aaaaggaatt attacaaggt
121 aaattggacc ttcttagtga taccaacatg gtagactttg ctatggatgt atacaaaaac
181 ctttattctg atgatattcc tcatgctttg agagagaaaa gaaccacagt ggttgcacaa
241 ctgaaacagc ttcaggcaga aacagaacca attgtgaaga tgtttgaaga tccagaaact
301 acaaggcaaa tgcagtcaac cagggatggt aggatgctct ttgactacct ggcggacaag
361 catggtttta ggcaggaata tttagataca ctctacagat atgcaaaatt ccagtacgaa
421 tgtgggaatt actcaggagc agcagaatat ctttattttt ttagagtgct ggttccagca
481 acagatagaa atgctttaag ttcactctgg ggaaagctgg cctctgaaat cttaatgcag
541 aattgggatg cagccatgga agaccttaca cggttaaaag agaccataga taataattct
601 gtgagttctc cacttcagtc tcttcagcag agaacatggc tcattcactg gtctctgttt
661 gttttcttca atcaccccaa aggtcgcgat aatattattg acctcttcct ttatcagcca
721 caatatctta atgcaattca gacaatgtgt ccacacattc ttcgctattt gactacagca
781 gtcataacaa acaaggatgt tcgaaaacgt cggctggttc taaaagatct agttaaagtt
841 attcaacagg agtcttacac atataaagac ccaattacag aatttgttga atgtttatat
901 gttaactttg actttgatgg ggctcagaaa aagctgaggg aatgtgaatc agtgcttgtg
961 aatgacttct tcttggtggc ttgtcttgag gatttcattg aaaatgcccg tctcttcata
1021 tttgagactt tctgtcgcat ccaccagtgt atcagcatta acatgttggc agataaattg
1081 aacatgactc cagaagaagc tgaaaggtgg attgtaaatt tgattagaaa tgcaagactg
1141 gatgccaaga ttgattctaa attaggtcat gtggttatgg gtaacaatgc agtctcaccc
1201 tatcagcaag tgattgaaaa gaccaaaagc ctttccttta gaagccagat gttggccatg
1261 aatattgaga agaaacttaa tcagaatagc aggtcagagg ctcctaactg ggcaactcaa
1321 gattctggct tctattaa
//