LOCUS CR457009 702 bp mRNA linear HUM 03-JUN-2004
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834G065D for
gene CRSP9, cofactor required for Sp1 transcriptional activation,
subunit 9, 33kDa; complete cds, incl. stopcodon.
ACCESSION CR457009
VERSION CR457009.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 702)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 702)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834G065D, ORFNo 1549
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834G065D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_004270 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..702
/db_xref="H-InvDB:HIT000267859"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834G065D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..702
/codon_start=1
/gene="CRSP9"
/db_xref="GOA:Q6IAZ5"
/db_xref="H-InvDB:HIT000267859.12"
/db_xref="InterPro:IPR009244"
/db_xref="InterPro:IPR037212"
/db_xref="UniProtKB/TrEMBL:Q6IAZ5"
/protein_id="CAG33290.1"
/translation="MGEPQQVSALPPPPMQYIKEYTDENIQEGLAPKPPPPIKDSYMM
FGNQFQCDDLIIRPLESQGIERLHPMQFDHKKELRKLNMSILINFLDLLDILIRSPGS
IKREEKLEDLKLLFVHVHHLINEYRPHQARETLRVMMEVQKRQRLETAERFQKHLERV
IEMIQNCLASLPDDLPHSEAGMRVKTEPMDADDSNNCTGQNEHQRENSGHRRDQIIEK
DAALCVLIDEMNERP"
BASE COUNT 242 a 133 c 149 g 178 t
ORIGIN
1 atgggtgaac cacagcaagt gagtgcactt ccaccacctc caatgcaata tatcaaggaa
61 tatacggatg aaaatattca agaaggctta gctcccaagc ctccccctcc aataaaagac
121 agttacatga tgtttggcaa tcagttccaa tgtgatgatc ttatcatccg ccctttggaa
181 agtcagggca tcgaacggct tcatcctatg cagtttgatc acaagaaaga actgagaaaa
241 cttaatatgt ctatccttat taatttcttg gaccttttag atattttaat aaggagccct
301 gggagtataa aacgagaaga gaaactagaa gatcttaagc tgctttttgt acacgtgcat
361 catcttataa atgaataccg accccaccaa gcaagagaga ccttgagagt catgatggag
421 gtccagaaac gtcaacggct tgaaacagct gagagatttc aaaagcacct ggaacgagta
481 attgaaatga ttcagaattg cttggcttct ttgcctgatg atttgcctca ttcagaagca
541 ggaatgagag taaaaactga accaatggat gctgatgata gcaacaattg tactggacag
601 aatgaacatc aaagagaaaa ttcaggtcat aggagagatc agattataga gaaagatgct
661 gccttgtgtg tcctaattga tgagatgaat gaaagacctt aa
//