LOCUS CR457005 231 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834F125D for gene NDUFC1, NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 1, 6kDa; complete cds, incl. stopcodon. ACCESSION CR457005 VERSION CR457005.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 231) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 231) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834F125D, ORFNo 1542 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834F125D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is a part of a collection of human full length expression clones generated by RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC (ATG). The last base of the last coding triplett has been changed to T, which might lead to an amino acid change at the C terminus of the polypeptide. The stop codon has been set to TAA followed by TTAACCCAGCTTTCTT..att. Compared to the reference sequence NM_002494 we found amino acid exchange(s) at position (first base of changed triplet): 226(glu->asp) Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..231 /db_xref="H-InvDB:HIT000267855" /organism="Homo sapiens" /lab_host="DH10B" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834F125D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..231 /codon_start=1 /gene="NDUFC1" /db_xref="GOA:Q6IAZ9" /db_xref="H-InvDB:HIT000267855.11" /db_xref="InterPro:IPR026192" /db_xref="UniProtKB/TrEMBL:Q6IAZ9" /protein_id="CAG33286.1" /translation="MAPSALLRPLSRLLAPARLPSGPSVRSKFYVREPPNAKPDWLKV GFTLGTTVFLWIYLIKQHNEDILEYKRRNGLD" BASE COUNT 53 a 67 c 58 g 53 t ORIGIN 1 atggcgccgt ccgccttgct gcgtcccctt tcccggctgc tggcccccgc caggctcccg 61 agcggccctt cagtgcgatc aaagttctac gtgcgagagc cgccgaatgc caaacctgac 121 tggctgaaag ttgggttcac cttgggcacc actgtcttct tgtggatcta tctcatcaaa 181 caacacaatg aagatatttt agagtacaaa agaagaaatg ggctggatta a //