LOCUS CR456998 894 bp mRNA linear HUM 03-JUN-2004
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834F015D for
gene TST, thiosulfate sulfurtransferase (rhodanese); complete cds,
incl. stopcodon.
ACCESSION CR456998
VERSION CR456998.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 894)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 894)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834F015D, ORFNo 1527
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834F015D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_003312 we found amino acid
exchange(s) at position (first base of changed triplet):
304(glu->asp)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..894
/db_xref="H-InvDB:HIT000267848"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834F015D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..894
/codon_start=1
/gene="TST"
/db_xref="GOA:Q16762"
/db_xref="H-InvDB:HIT000267848.12"
/db_xref="HGNC:HGNC:12388"
/db_xref="InterPro:IPR001307"
/db_xref="InterPro:IPR001763"
/db_xref="InterPro:IPR036873"
/db_xref="UniProtKB/Swiss-Prot:Q16762"
/protein_id="CAG33279.1"
/translation="MVHQVLYRALVSTKWLAESIRTGKLGPGLRVLDASWYSPGTREA
RKEYLERHVPGASFFDIEECRDTASPYEMMLPSEAGFAEYVGRLGISNHTHVVVYDGD
HLGSFYAPRVWWMFRVFGHRTVSVLNGGFRNWLKEGHPVTSEPSRPEPAVFKATLDRS
LLKTYEQVLENLESKRFQLVDSRSQGRFLGTEPEPDAVGLDSGHIRGAVNMPFMDFLT
EDGFEKGPEELRALFQTKKVDLSQPLIATCRKGVTACHVALAAYLCGKPDVAVYDGSW
SEWFRRAPPESRVSQGKSEKA"
BASE COUNT 161 a 273 c 285 g 175 t
ORIGIN
1 atggttcatc aggtgctcta ccgggcgctg gtctccacca agtggctggc ggagtccatc
61 aggactggca agctggggcc cggcctgcgg gtgctggacg cgtcctggta ctcaccaggc
121 acccgagagg cccgcaagga gtacctcgag cgccacgtac ccggcgcctc tttctttgac
181 atagaagagt gccgggacac ggcgtcgccc tacgagatga tgctgcccag cgaggctggc
241 ttcgccgagt atgtgggccg cctgggcatc agcaaccaca cgcacgtggt ggtgtatgat
301 ggtgaccacc tgggcagctt ctatgctccc cgggtctggt ggatgttccg tgtgtttggc
361 caccgcaccg tatcagtgct caatggtggc ttccggaact ggctgaagga gggccacccg
421 gtgacatccg agccctcacg cccagaaccg gccgtcttca aagccacact ggaccgctcc
481 ctgctcaaga cctacgagca ggtgctggag aaccttgaat ctaagaggtt ccagctggtg
541 gattcaaggt ctcaagggcg gttcctgggc accgagccgg agccggatgc agtaggactg
601 gactcgggcc atatccgtgg tgccgtcaac atgcctttca tggacttcct gactgaggat
661 ggcttcgaga agggcccaga agagctccgt gctctgttcc agaccaagaa ggtggatctc
721 tcgcagcctc tcattgccac gtgccgcaag ggagtcaccg cctgccacgt ggccttggct
781 gcctacctct gcggcaagcc tgatgtggcc gtgtacgatg gctcctggtc cgagtggttt
841 cgccgggccc ccccagagag ccgtgtgtcc cagggaaagt ctgagaaggc ttaa
//