LOCUS CR456992 465 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E045D for
gene ZNHIT1, zinc finger, HIT domain containing 1; complete cds,
incl. stopcodon.
ACCESSION CR456992
VERSION CR456992.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 465)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 465)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834E045D, ORFNo 1509
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E045D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_006349 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..465
/db_xref="H-InvDB:HIT000267842"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834E045D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..465
/codon_start=1
/gene="ZNHIT1"
/db_xref="GOA:O43257"
/db_xref="H-InvDB:HIT000267842.12"
/db_xref="HGNC:HGNC:21688"
/db_xref="InterPro:IPR007529"
/db_xref="InterPro:IPR039723"
/db_xref="UniProtKB/Swiss-Prot:O43257"
/protein_id="CAG33273.1"
/translation="MVEKKTSVRSQDPGQRRVLDRAARQRRINRQLEALENDNFQDDP
HAGLPQLGKRLPQFDDDADTGKKKKKTRGDHFKLRFRKNFQALLEEQNLSVAEGPNYL
TACAGPPSRPQRPFCAVCGFPSPYTCVSCGARYCTVRCLGTHQETRCLKWTV"
BASE COUNT 98 a 140 c 140 g 87 t
ORIGIN
1 atggtggaga agaaaacttc ggttcgctcc caggaccccg ggcagcggcg ggtgctggac
61 cgggctgccc ggcagcgtcg catcaaccgg cagctggagg ccctggagaa tgacaacttc
121 caggatgacc cccacgcggg actccctcag ctcggcaaga gactgcctca gtttgatgac
181 gatgcggaca ctggaaagaa aaagaagaaa acccgaggtg atcattttaa acttcgcttc
241 cgaaaaaact ttcaggccct gttggaggag cagaacttga gtgtggccga gggccctaac
301 tacctgacgg cctgtgcggg acccccatcg cggccccagc gccccttctg tgctgtctgt
361 ggcttcccat ccccctacac ctgtgtcagc tgcggtgccc ggtactgcac tgtgcgctgt
421 ctggggaccc accaggagac caggtgtctg aagtggactg tttaa
//