LOCUS CR456989 597 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E025D for
gene YKT6, SNARE protein Ykt6; complete cds, incl. stopcodon.
ACCESSION CR456989
VERSION CR456989.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 597)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 597)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834E025D, ORFNo 1504
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E025D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_006555 we found amino acid
exchange(s) at position (first base of changed triplet):
592(met->ile)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..597
/db_xref="H-InvDB:HIT000267839"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834E025D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..597
/codon_start=1
/gene="YKT6"
/db_xref="GOA:O15498"
/db_xref="H-InvDB:HIT000267839.11"
/db_xref="HGNC:HGNC:16959"
/db_xref="InterPro:IPR001388"
/db_xref="InterPro:IPR010908"
/db_xref="InterPro:IPR011012"
/db_xref="UniProtKB/Swiss-Prot:O15498"
/protein_id="CAG33270.1"
/translation="MKLYSLSVLYKGEAKVVLLKAAYDVSSFSFFQRSSVQEFMTFTS
QLIVERSSKGTRASVKEQDYLCHVYVRNDSLAGVVIADNEYPSRVAFTLLEKVLDEFS
KQVDRIDWPVGSPATIHYPALDGHLSRYQNPREADPMTKVQAELDETKIILHNTMESL
LERGEKLDDLVSKSEVLGTQSKAFYKTARKQNSCCAII"
BASE COUNT 167 a 152 c 142 g 136 t
ORIGIN
1 atgaagctgt acagcctcag cgtcctctac aaaggcgagg ccaaggtggt gctgctcaaa
61 gccgcatacg atgtgtcttc cttcagcttt ttccagagat ccagcgttca ggaattcatg
121 accttcacga gtcaactgat tgtggagcgc tcatcgaaag gcactagagc ttctgtcaaa
181 gaacaagact atctgtgcca cgtctacgtc cggaatgata gtcttgcagg tgtggtcatt
241 gctgacaatg aatacccatc ccgggtggcc tttaccttgc tggagaaggt actagatgaa
301 ttctccaagc aagtcgacag gatagactgg ccagtaggat cccctgctac aatccattac
361 ccagccctgg atggtcacct cagtagatac cagaacccac gagaagctga tcccatgact
421 aaagtgcagg ccgaactaga tgagaccaaa atcattctgc acaacaccat ggagtctctg
481 ttagagcgag gtgagaagct agatgacttg gtgtccaaat ccgaggtgct gggaacacag
541 tctaaagcct tctataaaac tgcccggaaa caaaactcat gctgtgccat catttaa
//