LOCUS CR456982 822 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D015D for gene PSMB10, proteasome (prosome, macropain) subunit, beta type, 10; complete cds, incl. stopcodon. ACCESSION CR456982 VERSION CR456982.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 822) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 822) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834D015D, ORFNo 1484 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D015D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is a part of a collection of human full length expression clones generated by RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC (ATG). The last base of the last coding triplett has been changed to T, which might lead to an amino acid change at the C terminus of the polypeptide. The stop codon has been set to TAA followed by TTAACCCAGCTTTCTT..att. Compared to the reference sequence BC017198 we found amino acid exchange(s) at position (first base of changed triplet): 46(asn->lys) 817(glu->asp) Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..822 /db_xref="H-InvDB:HIT000267832" /organism="Homo sapiens" /lab_host="DH10B" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834D015D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..822 /codon_start=1 /gene="PSMB10" /db_xref="GOA:Q6IB22" /db_xref="H-InvDB:HIT000267832.12" /db_xref="InterPro:IPR000243" /db_xref="InterPro:IPR001353" /db_xref="InterPro:IPR016050" /db_xref="InterPro:IPR023333" /db_xref="InterPro:IPR024689" /db_xref="InterPro:IPR029055" /db_xref="InterPro:IPR034384" /db_xref="UniProtKB/TrEMBL:Q6IB22" /protein_id="CAG33263.1" /translation="MLKPALEPRGGFSFEKCQRNASLERVLPGLKVPHARKTGTTIAG LVFQDGVILGADTRATNDSVVADKSCEKIHFIAPKIYCCGAGVAADAEMTTRMVASKM ELHALSTGREPRVATVTRILRQTLFRYQGHVGASLIVGGVDLTGPQLYGVHPHGSYSR LPFTALGSGQDAALAVLEDRFQPNMTLEAAQGLLVEAVTAGILGDLGSGGNVDACVIT KTGAKLLRTLSSPTEPVKRSGRYHFVPGTTAVLTQTVKPLTLELVEETVQAMEVD" BASE COUNT 164 a 253 c 263 g 142 t ORIGIN 1 atgctgaagc cagccctgga gccccgaggg ggcttctcct tcgagaaatg ccaaagaaat 61 gcatcattgg aacgcgtcct cccggggctc aaggtccctc acgcacgcaa gaccgggacc 121 accatcgcgg gcctggtgtt ccaagacggg gtcattctgg gcgccgatac gcgagccact 181 aacgattcgg tcgtggcgga caagagctgc gagaagatcc acttcatcgc ccccaaaatc 241 tactgctgtg gggctggagt agccgcggac gccgagatga ccacacggat ggtggcgtcc 301 aagatggagc tacacgcgct atctacgggc cgcgagcccc gcgtggccac ggtcactcgc 361 atcctgcgcc agacgctctt caggtaccag ggccacgtgg gtgcatcgct gatcgtgggc 421 ggcgtagacc tgactggacc gcagctctac ggtgtgcatc cccatggctc ctacagccgt 481 ctgcccttca cagccctggg ctctggtcag gacgcggccc tggcggtgct agaagaccgg 541 ttccagccga acatgacgct ggaggctgct caggggctgc tggtggaagc cgtcaccgcc 601 gggatcttgg gtgacctggg ctccgggggc aatgtggacg catgtgtgat cacaaagact 661 ggcgccaagc tgctgcggac actgagctca cccacagagc ccgtgaagag gtctggccgc 721 taccactttg tgcctggaac cacagctgtc ctgacccaga cagtgaagcc actaaccctg 781 gagctagtgg aggaaactgt gcaggctatg gaggtggatt aa //