LOCUS       CR456982                 822 bp    mRNA    linear   HUM 16-OCT-2008
DEFINITION  Homo sapiens full open reading frame cDNA clone RZPDo834D015D for
            gene PSMB10, proteasome (prosome, macropain) subunit, beta type,
            10; complete cds, incl. stopcodon.
ACCESSION   CR456982
VERSION     CR456982.1
KEYWORDS    Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
  TITLE     Cloning of human full open reading frames in Gateway(TM) system
            entry vector (pDONR201)
  JOURNAL   Unpublished.
REFERENCE   2  (bases 1 to 822)
  AUTHORS   Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
  JOURNAL   Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
            Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
            580, D-69120 Heidelberg, Germany
COMMENT     RZPD; RZPDo834D015D, ORFNo 1484
            www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D015D
            RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
            RZPD LIB No. 834
            www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
            www.rzpd.de/products/orfclones/
            Contact: Inge Arlart
            RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
            Heubnerweg 6, D-14059 Berlin, Germany
            Tel: +49 30 32639 100
            Fax: +49 30 32639 111
            www.rzpd.de
            This clone is available from RZPD;
            contact RZPD (customer.service@rzpd.de) for further information.
            
            This CDS clone is a part of a collection of human full length
            expression clones generated by RZPD.
            This CDS has been cloned incl. stopcodon.
            The CDS has been inserted into pDONR201 via a BP Clonase(TM)
            reaction.
            Additional sequence has been added in front of the start codon:
            att..AAAAAA GCA GGC (ATG).
            The last base of the last coding triplett has been changed to T,
            which might lead to an amino acid change at the C terminus of the
            polypeptide.
            The stop codon has been set to TAA followed by
            TTAACCCAGCTTTCTT..att.
            Compared to the reference sequence BC017198 we found amino acid
            exchange(s) at position (first base of changed triplet):
            46(asn->lys) 817(glu->asp)
            Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES             Location/Qualifiers
     source          1..822
                     /db_xref="H-InvDB:HIT000267832"
                     /organism="Homo sapiens"
                     /lab_host="DH10B"
                     /mol_type="mRNA"
                     /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
                     /clone="RZPDo834D015D"
                     /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
                     /db_xref="taxon:9606"
     CDS             1..822
                     /codon_start=1
                     /gene="PSMB10"
                     /db_xref="GOA:Q6IB22"
                     /db_xref="H-InvDB:HIT000267832.12"
                     /db_xref="InterPro:IPR000243"
                     /db_xref="InterPro:IPR001353"
                     /db_xref="InterPro:IPR016050"
                     /db_xref="InterPro:IPR023333"
                     /db_xref="InterPro:IPR024689"
                     /db_xref="InterPro:IPR029055"
                     /db_xref="InterPro:IPR034384"
                     /db_xref="UniProtKB/TrEMBL:Q6IB22"
                     /protein_id="CAG33263.1"
                     /translation="MLKPALEPRGGFSFEKCQRNASLERVLPGLKVPHARKTGTTIAG
                     LVFQDGVILGADTRATNDSVVADKSCEKIHFIAPKIYCCGAGVAADAEMTTRMVASKM
                     ELHALSTGREPRVATVTRILRQTLFRYQGHVGASLIVGGVDLTGPQLYGVHPHGSYSR
                     LPFTALGSGQDAALAVLEDRFQPNMTLEAAQGLLVEAVTAGILGDLGSGGNVDACVIT
                     KTGAKLLRTLSSPTEPVKRSGRYHFVPGTTAVLTQTVKPLTLELVEETVQAMEVD"
BASE COUNT          164 a          253 c          263 g          142 t
ORIGIN      
        1 atgctgaagc cagccctgga gccccgaggg ggcttctcct tcgagaaatg ccaaagaaat
       61 gcatcattgg aacgcgtcct cccggggctc aaggtccctc acgcacgcaa gaccgggacc
      121 accatcgcgg gcctggtgtt ccaagacggg gtcattctgg gcgccgatac gcgagccact
      181 aacgattcgg tcgtggcgga caagagctgc gagaagatcc acttcatcgc ccccaaaatc
      241 tactgctgtg gggctggagt agccgcggac gccgagatga ccacacggat ggtggcgtcc
      301 aagatggagc tacacgcgct atctacgggc cgcgagcccc gcgtggccac ggtcactcgc
      361 atcctgcgcc agacgctctt caggtaccag ggccacgtgg gtgcatcgct gatcgtgggc
      421 ggcgtagacc tgactggacc gcagctctac ggtgtgcatc cccatggctc ctacagccgt
      481 ctgcccttca cagccctggg ctctggtcag gacgcggccc tggcggtgct agaagaccgg
      541 ttccagccga acatgacgct ggaggctgct caggggctgc tggtggaagc cgtcaccgcc
      601 gggatcttgg gtgacctggg ctccgggggc aatgtggacg catgtgtgat cacaaagact
      661 ggcgccaagc tgctgcggac actgagctca cccacagagc ccgtgaagag gtctggccgc
      721 taccactttg tgcctggaac cacagctgtc ctgacccaga cagtgaagcc actaaccctg
      781 gagctagtgg aggaaactgt gcaggctatg gaggtggatt aa
//