LOCUS CR456967 429 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B095D for gene GMFG, glia maturation factor, gamma; complete cds, incl. stopcodon. ACCESSION CR456967 VERSION CR456967.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 429) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 429) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834B095D, ORFNo 1451 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B095D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is a part of a collection of human full length expression clones generated by RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC (ATG). The last base of the last coding triplett has been changed to T, which might lead to an amino acid change at the C terminus of the polypeptide. The stop codon has been set to TAA followed by TTAACCCAGCTTTCTT..att. Compared to the reference sequence NM_004877 we did not find any amino acid exchanges. Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..429 /db_xref="H-InvDB:HIT000267817" /organism="Homo sapiens" /lab_host="DH10B" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834B095D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..429 /codon_start=1 /gene="GMFG" /db_xref="GOA:O60234" /db_xref="H-InvDB:HIT000267817.13" /db_xref="HGNC:HGNC:4374" /db_xref="InterPro:IPR002108" /db_xref="InterPro:IPR011171" /db_xref="InterPro:IPR029006" /db_xref="InterPro:IPR030076" /db_xref="PDB:3L50" /db_xref="UniProtKB/Swiss-Prot:O60234" /protein_id="CAG33248.1" /translation="MSDSLVVCEVDPELTEKLRKFRFRKETDNAAIIMKVDKDRQMVV LEEEFQNISPEELKMELPERQPRFVVYSYKYVHDDGRVSYPLCFIFSSPVGCKPEQQM MYAGSKNRLVQTAELTKVFEIRTTDDLTEAWLQEKLSFFR" BASE COUNT 121 a 98 c 119 g 91 t ORIGIN 1 atgtctgact ccctggtggt gtgcgaggta gacccagagc taacagaaaa gctgaggaaa 61 ttccgcttcc gaaaagagac agacaatgca gccatcataa tgaaggtgga caaagaccgg 121 cagatggtgg tgctggagga agaatttcag aacatttccc cagaggagct caaaatggag 181 ttgccggaga gacagcccag gttcgtggtt tacagctaca agtacgtgca tgacgatggc 241 cgagtgtcct accctttgtg tttcatcttc tccagccctg tgggctgcaa gccggaacaa 301 cagatgatgt atgcagggag taaaaacagg ctggtgcaga cagcagagct cacaaaggtg 361 ttcgaaatcc gcaccactga tgacctcact gaggcctggc tccaagaaaa gttgtctttc 421 tttcgttaa //