LOCUS CR456964 852 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B065D for
gene VDAC2, voltage-dependent anion channel 2; complete cds, incl.
stopcodon.
ACCESSION CR456964
VERSION CR456964.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 852)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 852)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B065D, ORFNo 1447
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B065D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence BC012883 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..852
/db_xref="H-InvDB:HIT000267814"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B065D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..852
/codon_start=1
/gene="VDAC2"
/db_xref="GOA:P45880"
/db_xref="H-InvDB:HIT000267814.12"
/db_xref="HGNC:HGNC:12672"
/db_xref="InterPro:IPR001925"
/db_xref="InterPro:IPR023614"
/db_xref="InterPro:IPR027246"
/db_xref="InterPro:IPR030277"
/db_xref="UniProtKB/Swiss-Prot:P45880"
/protein_id="CAG33245.1"
/translation="MCIPPSYADLGKAARDIFNKGFGFGLVKLDVKTKSCSGVEFSTS
GSSNTDTGKVTGTLETKYKWCEYGLTFTEKWNTDNTLGTEIAIEDQICQGLKLTFDTT
FSPNTGKKSGKIKSSYKRECINLGCDVDFDFAGPAIHGSAVFGYEGWLAGYQMTFDSA
KSKLTRNNFAVGYRTGDFQLHTNVNDGTEFGGSIYQKVCEDLDTSVNLAWTSGTNCTR
FGIAAKYQLDPTASISAKVNNSSLIGVGYTQTLRPGVKLTLSALVDGKSINAGGHKVG
LALELEA"
BASE COUNT 242 a 164 c 209 g 237 t
ORIGIN
1 atgtgtattc ctccatcata tgctgacctt ggcaaagctg ccagagatat tttcaacaaa
61 ggatttggtt ttgggttggt gaaactggat gtgaaaacaa agtcttgcag tggcgtggaa
121 ttttcaacgt ccggttcatc taatacagac actggtaaag ttactgggac cttggagacc
181 aaatacaagt ggtgtgagta tggtctgact ttcacagaaa agtggaacac tgataacact
241 ctgggaacag aaatcgcaat tgaagaccag atttgtcaag gtttgaaact gacatttgat
301 actaccttct caccaaacac aggaaagaaa agtggtaaaa tcaagtcttc ttacaagagg
361 gagtgtataa accttggttg tgatgttgac tttgattttg ctggacctgc aatccatggt
421 tcagctgtct ttggttatga gggctggctt gctggctacc agatgacctt tgacagtgcc
481 aaatcaaagc tgacaaggaa taactttgca gtgggctaca ggactgggga cttccagcta
541 cacactaatg tcaatgatgg gacagaattt ggaggatcaa tttatcagaa agtttgtgaa
601 gatcttgaca cttcagtaaa ccttgcttgg acatcaggta ccaactgcac tcgttttggc
661 attgcagcta aatatcagtt ggatcccact gcttccattt ctgcaaaagt caacaactct
721 agcttaattg gagtaggcta tactcagact ctgaggcctg gtgtgaagct tacactctct
781 gctctggtag atgggaagag cattaatgct ggaggccaca aggttgggct cgccctggag
841 ttggaggctt aa
//