LOCUS CR456962 501 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B025D for
gene MYL2, myosin, light polypeptide 2, regulatory, cardiac, slow;
complete cds, incl. stopcodon.
ACCESSION CR456962
VERSION CR456962.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 501)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 501)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B025D, ORFNo 1436
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B025D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence BC031006 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..501
/db_xref="H-InvDB:HIT000267812"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B025D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..501
/codon_start=1
/gene="MYL2"
/db_xref="GOA:Q6IB42"
/db_xref="H-InvDB:HIT000267812.12"
/db_xref="InterPro:IPR002048"
/db_xref="InterPro:IPR011992"
/db_xref="InterPro:IPR018247"
/db_xref="UniProtKB/TrEMBL:Q6IB42"
/protein_id="CAG33243.1"
/translation="MAPKKAKKRAGGANSNVFSMFEQTQIQEFKEAFTIMDQNRDGFI
DKNDLRDTFAALGRVNVKNEEIDEMIKEAPGPINFTVFLTMFGEKLKGADPEETILNA
FKVFDPEGKGVLKADYVREMLTTQAERFSKEEVDQMFAAFPPDVTGNLDYKNLVHIIT
HGEEKD"
BASE COUNT 149 a 114 c 137 g 101 t
ORIGIN
1 atggcaccta agaaagcaaa gaagagagcc gggggcgcca actccaacgt gttctccatg
61 ttcgaacaga cccaaatcca ggaatttaag gaggccttca ctatcatgga ccagaacagg
121 gatggcttca ttgacaagaa cgatctgaga gacacctttg ctgcccttgg gcgagtgaac
181 gtgaaaaatg aagaaattga tgaaatgatc aaggaggctc cgggtccaat taactttact
241 gtgttcctca caatgtttgg ggagaaactt aagggagcgg accctgagga aaccattctc
301 aacgcattca aagtgtttga ccctgaaggc aaaggggtgc tgaaggctga ttacgttcgg
361 gaaatgctga ccacgcaggc ggagaggttt tccaaggagg aggttgacca gatgttcgcc
421 gccttccccc ctgacgtgac tggcaacttg gactacaaga acctggtgca catcatcacc
481 cacggagaag agaaggatta a
//