LOCUS CR456961 861 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B0119D for
gene SSR1, signal sequence receptor, alpha (translocon-associated
protein alpha); complete cds, incl. stopcodon.
ACCESSION CR456961
VERSION CR456961.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 861)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 861)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B0119D, ORFNo 1430
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B0119D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence BC007710 we found amino acid
exchange(s) at position (first base of changed triplet):
856(glu->asp)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..861
/db_xref="H-InvDB:HIT000267811"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B0119D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..861
/codon_start=1
/gene="SSR1"
/db_xref="GOA:P43307"
/db_xref="H-InvDB:HIT000267811.12"
/db_xref="HGNC:HGNC:11323"
/db_xref="InterPro:IPR005595"
/db_xref="UniProtKB/Swiss-Prot:P43307"
/protein_id="CAG33242.1"
/translation="MRLLPRLLLLLLLVFPATVLFRGGPRGLLAVAQDLTEDEETVED
SIIEDEDDEAEVEEDEPTDLVEDKEEEDVSGEPEASPSADTTILFVKGEDFPANNIVK
FLVGFTNKGTEDFIVESLDASFRYPQDYQFYIQNFTALPLNTVVPPQRQATFEYSFIP
AEPMGGRPFGLVINLNYKDLNGNVFQDAVFNQTVTVIEREDGLDGETIFMYMFLAGLG
LLVIVGLHQLLESRKRKRPIQKVEMGTSSQNDVDMSWIPQETLNQINKASPRRLPRKR
AQKRSVGSDD"
BASE COUNT 261 a 171 c 200 g 229 t
ORIGIN
1 atgagactcc tcccccgctt gctgctgctt ctcttactcg tgttccctgc cactgtcttg
61 ttccgaggcg gccccagagg cttgttagca gtggcacaag atcttacaga ggatgaagaa
121 acagtagaag attccataat tgaggatgaa gatgatgaag ccgaggtaga agaagatgaa
181 cccacagatt tggtagaaga taaagaggaa gaagatgtgt ctggtgaacc tgaagcttca
241 ccgagtgcag atacaactat actgtttgta aaaggagaag attttccagc aaataacatt
301 gtgaagttcc tggtaggctt taccaacaag ggtacagaag attttattgt tgaatcctta
361 gatgcctcat tccgttatcc tcaggactac cagttttata tccagaattt cacagctctt
421 cctctgaaca ctgtagtgcc accccagaga caggcaactt ttgagtactc tttcattcct
481 gcagagccca tgggcggacg accatttggt ttggtcatca atctgaacta caaagatttg
541 aacggcaatg tattccaaga tgcagtcttc aatcaaacag ttacagttat tgaaagagag
601 gatgggttag atggagaaac aatctttatg tatatgttcc ttgctggtct tgggcttctg
661 gttattgttg gccttcatca actcctagaa tctagaaagc gtaagagacc catacagaaa
721 gtagaaatgg gtacatcaag tcagaatgat gttgacatga gttggattcc tcaggaaaca
781 ttgaatcaaa tcaataaagc ttcaccaaga aggttgccca ggaaacgggc acagaagaga
841 tcagtgggat ctgatgatta a
//