LOCUS CR456957 1527 bp mRNA linear HUM 03-JUN-2004
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E0121D for
gene KIAA0020, KIAA0020; complete cds, incl. stopcodon.
ACCESSION CR456957
VERSION CR456957.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1527)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1527)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834E0121D, ORFNo 1408
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E0121D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_014878 we found amino acid
exchange(s) at position (first base of changed triplet):
1453(leu->ser)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1527
/db_xref="H-InvDB:HIT000267807"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834E0121D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1527
/codon_start=1
/gene="KIAA0020"
/db_xref="GOA:Q15397"
/db_xref="H-InvDB:HIT000267807.14"
/db_xref="HGNC:HGNC:29676"
/db_xref="InterPro:IPR001313"
/db_xref="InterPro:IPR011989"
/db_xref="InterPro:IPR012959"
/db_xref="InterPro:IPR016024"
/db_xref="InterPro:IPR033133"
/db_xref="InterPro:IPR040059"
/db_xref="PDB:4WZR"
/db_xref="PDB:4WZW"
/db_xref="UniProtKB/Swiss-Prot:Q15397"
/protein_id="CAG33238.1"
/translation="MWEILRRKDCDKEKRVKLMSDLQKLIQGKIKTIAFAHDSTRVIQ
CYIQYGNEEQRKQAFEELRDDLVELSKAKYSRNIVKKFLMYGSKPQIAEIIRSFKGHV
RKMLRHAEASAIVEYAYNDKAILEQRNMLTEELYGNTFQLYKSADHRTLDKVLEVQPE
KLELIMDEMKQILTPMAQKEAVIKHSLVHKVFLDFFTYAPPKLRSEMIEAIREAVVYL
AHTHDGARVAMHCLWHGTPKDRKVIVKTMKTYVEKVANGQYSHLVLLAAFDCIDDTKL
VKQIIISEIISSLPSIVNDKYGRKVLLYLLSPRDPAHTVREIIEVLQKGDGNAHSKKD
TEVRRRELLESISPALLSYLQEHAQEVVLDKSACVLVSDILGSATGDVQPTMNAIASL
AATGLHPGGKDGELHIAEHPAGHLVLKWLIEQDKKMKENGREGCFAKTLVEHVGMKNL
KSWASVNRGAIILSSLLQSCDLEVANKVKAALKSSIPTLEKTKSTSKGIEILLEKLST
"
BASE COUNT 515 a 283 c 365 g 364 t
ORIGIN
1 atgtgggaga ttttaagaag aaaagactgt gacaaagaaa aaagagtaaa gttaatgagt
61 gatttgcaga agttgattca agggaaaatt aaaactattg catttgcaca cgattcaact
121 cgtgtgatcc agtgttacat tcagtatggt aatgaagaac agagaaaaca ggcttttgaa
181 gaattgcgag atgatttggt tgagttaagt aaagccaaat attcgagaaa tattgttaag
241 aaatttctca tgtatggaag taaaccacag attgcagaga taatcagaag ttttaaaggc
301 cacgtgagga agatgctgcg gcatgcggaa gcatcagcca tcgtggagta cgcatacaat
361 gacaaagcca ttttggagca gaggaacatg ctgacggaag agctctatgg gaacacattt
421 cagctttaca agtcagcaga tcaccgaact ctggacaaag tgttagaggt acagccagaa
481 aaattagaac ttattatgga tgaaatgaaa cagattctaa ctccaatggc ccaaaaggaa
541 gctgtgatta agcactcatt ggtgcataaa gtattcttgg acttttttac ctatgcaccc
601 cccaaactca gatcagaaat gattgaagcc atccgcgaag cggtggtcta cctggcacac
661 acacacgatg gcgccagagt ggccatgcac tgcctgtggc atggcacgcc caaggacagg
721 aaagtgattg tgaaaacaat gaagacttat gttgaaaagg tggctaatgg ccaatactcc
781 catttggttt tactggcggc atttgattgt attgatgata ctaagcttgt gaagcagata
841 atcatatcag aaattatcag ttcattgcct agcatagtaa atgacaaata tggaaggaag
901 gtcctattgt acttactaag ccccagagat cctgcacata cagtacgaga aatcattgaa
961 gttctgcaaa aaggagatgg aaatgcacac agtaagaaag atacagaggt ccgcagacgg
1021 gagctcctag aatccatttc tccagctttg ttaagctacc tgcaagaaca cgcccaagaa
1081 gtggtgctag ataagtctgc gtgtgtgttg gtgtctgaca ttctgggatc tgccactgga
1141 gacgttcagc ctaccatgaa tgccatcgcc agcttggcag caacaggact gcatcctggt
1201 ggcaaggacg gagagcttca cattgcagaa catcctgcag gacatctagt tctgaagtgg
1261 ttaatagagc aagataaaaa gatgaaagaa aatgggagag aaggttgttt tgcaaaaaca
1321 cttgtagagc atgttggtat gaagaacctg aagtcctggg ctagtgtaaa tcgaggtgcc
1381 attattcttt ctagcctcct ccagagttgt gacctggaag ttgcaaacaa agtcaaagct
1441 gcactgaaaa gctcgattcc tacactggaa aaaaccaaaa gcaccagcaa aggaatagaa
1501 attctacttg aaaaactgag cacttaa
//