LOCUS CR456950 327 bp mRNA linear HUM 03-JUN-2004 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834C0516D for gene ATP5J, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6; complete cds, incl. stopcodon. ACCESSION CR456950 VERSION CR456950.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 327) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 327) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834C0516D, ORFNo 1386 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834C0516D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is a part of a collection of human full length expression clones generated by RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC (ATG). The last base of the last coding triplett has been changed to T, which might lead to an amino acid change at the C terminus of the polypeptide. The stop codon has been set to TAA followed by TTAACCCAGCTTTCTT..att. Compared to the reference sequence NM_001685 we did not find any amino acid exchanges. Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..327 /db_xref="H-InvDB:HIT000267800" /organism="Homo sapiens" /lab_host="DH10B" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834C0516D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..327 /codon_start=1 /gene="ATP5J" /db_xref="GOA:Q6IB54" /db_xref="H-InvDB:HIT000267800.13" /db_xref="InterPro:IPR008387" /db_xref="InterPro:IPR036204" /db_xref="UniProtKB/TrEMBL:Q6IB54" /protein_id="CAG33231.1" /translation="MILQRLFRFSSVIRSAVSVHLRRNIGVTAVAFNKELDPIQKLFV DKIREYKSKRQTSGGPVDASSEYQQELERELFKLKQMFGNADMNTFPTFKFEDPKFEV IEKPQA" BASE COUNT 99 a 66 c 72 g 90 t ORIGIN 1 atgattcttc agaggctctt caggttctcc tctgtcattc ggtcagccgt ctcagtccat 61 ttgcggagga acattggtgt tacagcagtg gcatttaata aggaacttga tcctatacag 121 aaactctttg tggacaagat tagagaatac aaatctaagc gacagacatc tggaggacct 181 gttgatgcta gttcagagta tcagcaagag ctggagaggg agctttttaa gctcaagcaa 241 atgtttggta atgcagacat gaatacattt cccaccttca aatttgaaga tcccaaattt 301 gaagtcatcg aaaaacccca ggcttaa //