LOCUS CR456947 1239 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E1218D for
gene CTSD, cathepsin D (lysosomal aspartyl protease); complete cds,
incl. stopcodon.
ACCESSION CR456947
VERSION CR456947.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1239)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1239)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834E1218D, ORFNo 1349
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E1218D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_001909 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1239
/db_xref="H-InvDB:HIT000267797"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834E1218D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1239
/codon_start=1
/gene="CTSD"
/db_xref="GOA:P07339"
/db_xref="H-InvDB:HIT000267797.12"
/db_xref="HGNC:HGNC:2529"
/db_xref="InterPro:IPR001461"
/db_xref="InterPro:IPR001969"
/db_xref="InterPro:IPR012848"
/db_xref="InterPro:IPR021109"
/db_xref="InterPro:IPR033121"
/db_xref="InterPro:IPR033144"
/db_xref="PDB:1LYA"
/db_xref="PDB:1LYB"
/db_xref="PDB:1LYW"
/db_xref="PDB:4OBZ"
/db_xref="PDB:4OC6"
/db_xref="PDB:4OD9"
/db_xref="UniProtKB/Swiss-Prot:P07339"
/protein_id="CAG33228.1"
/translation="MQPSSLLPLALCLLAAPASALVRIPLHKFTSIRRTMSEVGGSVE
DLIAKGPVSKYSQAVPAVTEGPIPEVLKNYMDAQYYGEIGIGTPPQCFTVVFDTGSSN
LWVPSIHCKLLDIACWIHHKYNSDKSSTYVKNGTSFDIHYGSGSLSGYLSQDTVSVPC
QSASSASALGGVKVERQVFGEATKQPGITFIAAKFDGILGMAYPRISVNNVLPVFDNL
MQQKLVDQNIFSFYLSRDPDAQPGGELMLGGTDSKYYKGSLSYLNVTRKAYWQVHLDQ
VEVASGLTLCKEGCEAIVDTGTSLMVGPVDEVRELQKAIGAVPLIQGEYMIPCEKVST
LPAITLKLGGKGYKLSPEDYTLKVSQAGKTLCLSGFMGMDIPPPSGPLWILGDVFIGR
YYTVFDRDNNRVGFAEAARL"
BASE COUNT 237 a 403 c 366 g 233 t
ORIGIN
1 atgcagccct ccagccttct gccgctcgcc ctctgcctgc tggctgcacc cgcctccgcg
61 ctcgtcagga tcccgctgca caagttcacg tccatccgcc ggaccatgtc ggaggttggg
121 ggctctgtgg aggacctgat tgccaaaggc cccgtctcaa agtactccca ggcggtgcca
181 gccgtgaccg aggggcccat tcccgaggtg ctcaagaact acatggacgc ccagtactac
241 ggggagattg gcatcgggac gcccccccag tgcttcacag tcgtcttcga cacgggctcc
301 tccaacctgt gggtcccctc catccactgc aaactgctgg acatcgcttg ctggatccac
361 cacaagtaca acagcgacaa gtccagcacc tacgtgaaga atggtacctc gtttgacatc
421 cactatggct cgggcagcct ctccgggtac ctgagccagg acactgtgtc ggtgccctgc
481 cagtcagcgt cgtcagcctc tgccctgggc ggtgtcaaag tggagaggca ggtctttggg
541 gaggccacca agcagccagg catcaccttc atcgcagcca agttcgatgg catcctgggc
601 atggcctacc cccgcatctc cgtcaacaac gtgctgcccg tcttcgacaa cctgatgcag
661 cagaagctgg tggaccagaa catcttctcc ttctacctga gcagggaccc agatgcgcag
721 cctgggggtg agctgatgct gggtggcaca gactccaagt attacaaggg ttctctgtcc
781 tacctgaatg tcacccgcaa ggcctactgg caggtccacc tggaccaggt ggaggtggcc
841 agcgggctga ccctgtgcaa ggagggctgt gaggccattg tggacacagg cacttccctc
901 atggtgggcc cggtggatga ggtgcgcgag ctgcagaagg ccatcggggc cgtgccgctg
961 attcagggcg agtacatgat cccctgtgag aaggtgtcca ccctgcccgc gatcacactg
1021 aagctgggag gcaaaggcta caagctgtcc ccagaggact acacgctcaa ggtgtcgcag
1081 gccgggaaga ccctctgcct gagcggcttc atgggcatgg acatcccgcc acccagcggg
1141 ccactctgga tcctgggcga cgtcttcatc ggccgctact acactgtgtt tgaccgtgac
1201 aacaacaggg tgggcttcgc cgaggctgcc cgcctttaa
//