LOCUS CR456942 546 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834A085D for
gene ISG20, interferon stimulated gene 20kDa; complete cds, incl.
stopcodon.
ACCESSION CR456942
VERSION CR456942.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 546)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 546)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834A085D, ORFNo 1328
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A085D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_002201 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..546
/db_xref="H-InvDB:HIT000267792"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834A085D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..546
/codon_start=1
/gene="ISG20"
/db_xref="GOA:Q96AZ6"
/db_xref="H-InvDB:HIT000267792.12"
/db_xref="HGNC:HGNC:6130"
/db_xref="InterPro:IPR012337"
/db_xref="InterPro:IPR013520"
/db_xref="InterPro:IPR036397"
/db_xref="PDB:1WLJ"
/db_xref="UniProtKB/Swiss-Prot:Q96AZ6"
/protein_id="CAG33223.1"
/translation="MAGSREVVAMDCEMVGLGPHRESGLARCSLVNVHGAVLYDKFIR
PEGEITDYRTRVSGVTPQHMVGATPFAVARLEILQLLKGKLVVGHDLKHDFQALKEDM
SGYTIYDTSTDRLLWREAKLDHCRRVSLRVLSERLLHKSIQNSLLGHSSVEDARATME
LYQISQRIRARRGLPRLAVSD"
BASE COUNT 114 a 153 c 183 g 96 t
ORIGIN
1 atggctggga gccgtgaggt ggtggccatg gactgcgaga tggtggggct ggggccccac
61 cgggagagtg gcctggctcg ttgcagcctc gtgaacgtcc acggtgctgt gctgtacgac
121 aagttcatcc ggcctgaggg agagatcacc gattacagaa cccgggtcag cggggtcacc
181 cctcagcaca tggtgggggc cacaccattt gccgtggcca ggctagagat cctgcagctc
241 ctgaaaggca agctggtggt gggtcatgac ctgaagcacg acttccaggc actgaaagag
301 gacatgagcg gctacacaat ctacgacacg tccactgaca ggctgttgtg gcgtgaggcc
361 aagctggacc actgcaggcg tgtctccctg cgggtgctga gtgagcgcct cctgcacaag
421 agcatccaga acagcctgct tggacacagc tcggtggaag atgcgagggc aacgatggag
481 ctctatcaaa tctcccagag aatccgagcc cgccgagggc tgccccgcct ggctgtgtca
541 gattaa
//