LOCUS CR456935 681 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834A0316D for
gene APACD, ATP binding protein associated with cell
differentiation; complete cds, incl. stopcodon.
ACCESSION CR456935
VERSION CR456935.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 681)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 681)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834A0316D, ORFNo 1317
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834A0316D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_005783 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..681
/db_xref="H-InvDB:HIT000267785"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834A0316D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..681
/codon_start=1
/gene="APACD"
/db_xref="GOA:O14530"
/db_xref="H-InvDB:HIT000267785.11"
/db_xref="HGNC:HGNC:24110"
/db_xref="InterPro:IPR013766"
/db_xref="InterPro:IPR036249"
/db_xref="UniProtKB/Swiss-Prot:O14530"
/protein_id="CAG33216.1"
/translation="MEADASVDMFSKVLEHQLLQTTKLVEEHLDSEIQKLDQMDEDEL
ERLKEKRLQALRKAQQQKQEWLSKGHGEYREIPSERDFFQEVKESENVVCHFYRDSTF
RCKILDRHLAILSKKHLETKFLKLNVEKAPFLCERLHIKVIPTLALLKDGKTQDYVVG
FTDLGNTDDFTTETLEWRLGSSDILNYSGNLMEPPFQNQKKFGTNFTKLEKKTIRGKK
YDSDSDDD"
BASE COUNT 248 a 122 c 146 g 165 t
ORIGIN
1 atggaagctg atgcatctgt tgacatgttt tccaaagtcc tggagcatca gctgcttcag
61 actaccaaac tggtggaaga acatttggat tctgaaattc aaaaactgga tcagatggat
121 gaggatgaat tggaacgcct taaagaaaag agactccagg cactaaggaa agctcaacag
181 cagaaacaag aatggctttc taaaggacat ggggaataca gagaaatccc tagtgaaaga
241 gacttttttc aagaagtcaa ggagagtgaa aatgtggttt gccatttcta cagagactcc
301 acattcaggt gtaaaatact agacagacat ctggcaatat tgtccaagaa acacctcgag
361 accaaatttt tgaagctgaa tgtggaaaaa gcacctttcc tttgtgagag actgcatatc
421 aaagtcattc ccacactagc actgctaaaa gatgggaaaa cacaagatta tgttgttggg
481 tttactgacc taggaaatac agatgacttc accacagaaa ctttagaatg gaggctcggt
541 tcttctgaca ttcttaatta cagtggaaat ttaatggagc caccatttca gaaccaaaag
601 aaatttggaa caaacttcac aaagctggaa aagaaaacta tccgaggaaa gaaatatgat
661 tcagactctg atgatgatta a
//