LOCUS CR456922 1872 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834D1221D for
gene XPNPEP1, X-prolyl aminopeptidase (aminopeptidase P) 1,
soluble; complete cds, incl. stopcodon.
ACCESSION CR456922
VERSION CR456922.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1872)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1872)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834D1221D, ORFNo 1293
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834D1221D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_020383 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1872
/db_xref="H-InvDB:HIT000267772"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834D1221D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1872
/codon_start=1
/gene="XPNPEP1"
/db_xref="GOA:Q9NQW7"
/db_xref="H-InvDB:HIT000267772.11"
/db_xref="HGNC:HGNC:12822"
/db_xref="InterPro:IPR000587"
/db_xref="InterPro:IPR000994"
/db_xref="InterPro:IPR001131"
/db_xref="InterPro:IPR029149"
/db_xref="InterPro:IPR032416"
/db_xref="InterPro:IPR033740"
/db_xref="InterPro:IPR036005"
/db_xref="PDB:3CTZ"
/db_xref="UniProtKB/Swiss-Prot:Q9NQW7"
/protein_id="CAG33203.1"
/translation="MPPKVTSELLRQLRQAMRNSEYVTEPIQAYIIPSGDAHQSEYIA
PCDCRRAFVSGFDGSAGTAIITEEHAAMWTDGRYFLQAAKQMDSNWTLMKMGLKDTPT
QEDWLVSVLPEGSRVGVDPLIIPTDYWKKMAKVLRSAGHHLIPVKENLVDKIWTDRPE
RPCKPLLTLGLDYTGISWKDKVADLRLKMAERNVMWFVVTALDEIAWLFNLRGSDVEH
NPVFFSYAIIGLETIMLFIDGDRIDAPSVKEHLLLDLGLEAEYRIQVHPYKSILSELK
ALCADLSPREKVWVSDKASYAVSETIPKDHRCCMPYTPICIAKAVKNSAESEGMRRAH
IKDAVALCELFNWLEKEVPKGGVTEISAADKAEEFRRQQADFVDLSFPTISSTGPNGA
IIHYAPVPETNRTLSLDEVYLIDSGAQYKDGTTDVTRTMHFGTPTAYEKECFTYVLKG
HIAVSAAVFPTGTKGHLLDSFARSALWDSGLDYLHGTGHGVGSFLNVHEGPCGISYKT
FSDEPLEAGMIVTDEPGYYEDGAFGIRIENVVLVVPVKTKYNFNNRGSLTFEPLTLVP
IQTKMIDVDSLTDKECDWLNNYHLTCRDVIGKELQKQGRQEALEWLIRETQPISKQH"
BASE COUNT 468 a 464 c 498 g 442 t
ORIGIN
1 atgcctccaa aggtgacttc agagctgctt cggcagctga gacaagccat gaggaactct
61 gagtatgtga ccgaaccgat ccaggcctac atcatcccat cgggagatgc tcatcagagt
121 gagtatattg ctccatgtga ctgtcggcgg gcttttgtct ctggattcga tggctctgcg
181 ggcacagcca tcatcacaga agagcatgca gccatgtgga ctgacgggcg ctactttctc
241 caggctgcca agcaaatgga cagcaactgg acacttatga agatgggtct gaaggacaca
301 ccaactcagg aagactggct ggtgagtgtg cttcctgaag gatccagggt tggtgtggac
361 cccttgatca ttcctacaga ttattggaag aaaatggcca aagttctgag aagtgccggc
421 catcacctca ttcctgtcaa ggagaacctc gttgacaaaa tctggacaga ccgtcctgag
481 cgcccttgca agcctctcct cacactgggc ctggattaca caggcatctc ctggaaggac
541 aaggttgcag accttcggtt gaaaatggct gagaggaacg tcatgtggtt tgtggtcact
601 gccttggatg agattgcgtg gctatttaat ctccgaggat cagatgtgga gcacaatcca
661 gtatttttct cctacgcaat cataggacta gagacgatca tgctcttcat tgatggtgac
721 cgcatagacg cccccagtgt gaaggagcac ctgcttcttg acttgggtct ggaagccgaa
781 tacaggatcc aggtgcatcc ctacaagtcc atcctgagcg agctcaaggc cctgtgtgct
841 gacctctccc caagggagaa ggtgtgggtc agtgacaagg ccagctatgc tgtgagcgag
901 accatcccca aggaccaccg ctgctgtatg ccttacaccc ccatctgcat cgccaaagct
961 gtgaagaatt cagctgagtc agaaggcatg aggcgggctc acattaaaga tgctgttgct
1021 ctctgtgaac tctttaactg gctggagaaa gaggttccca aaggtggtgt gacagagatc
1081 tcagctgctg acaaagctga ggagtttcgc aggcaacagg cagactttgt ggacctgagc
1141 ttcccaacaa tttccagtac gggacccaac ggcgccatca ttcactacgc gccagtccct
1201 gagacgaata ggaccttgtc cctggatgag gtgtacctta ttgactcggg tgctcaatac
1261 aaggatggca ccacagatgt gacgcggaca atgcattttg ggacccctac agcctacgag
1321 aaggaatgct tcacatatgt cctcaagggc cacatagctg tgagtgcagc cgttttcccg
1381 actggaacca aaggtcacct tcttgactcc tttgcccgtt cagctttatg ggattcaggc
1441 ctagattact tgcacgggac tggacatggt gttgggtctt ttttgaatgt ccatgagggt
1501 ccttgcggca tcagttacaa aacattctct gatgagccct tggaggcagg catgattgtc
1561 actgatgagc ccgggtacta tgaagatggg gcttttggaa ttcgcattga gaatgttgtc
1621 cttgtggttc ctgtgaagac caagtataat tttaataacc ggggaagcct gacctttgaa
1681 cctctaacat tggttccaat tcagaccaaa atgatagatg tggattctct tacagacaaa
1741 gagtgcgact ggctcaacaa ttaccacctg acctgcaggg atgtgattgg gaaggaattg
1801 cagaaacagg gccgccagga agctctcgag tggctcatca gagagacgca acccatctcc
1861 aaacagcatt aa
//