LOCUS CR456921 1239 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834E1118D for
gene BHLHB2, basic helix-loop-helix domain containing, class B, 2;
complete cds, incl. stopcodon.
ACCESSION CR456921
VERSION CR456921.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1239)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1239)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834E1118D, ORFNo 1292
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834E1118D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_003670 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1239
/db_xref="H-InvDB:HIT000267771"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834E1118D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1239
/codon_start=1
/gene="BHLHB2"
/db_xref="GOA:Q6IB83"
/db_xref="H-InvDB:HIT000267771.12"
/db_xref="InterPro:IPR003650"
/db_xref="InterPro:IPR011598"
/db_xref="InterPro:IPR036638"
/db_xref="UniProtKB/TrEMBL:Q6IB83"
/protein_id="CAG33202.1"
/translation="MERIPSAQPPPACLPKAPGLEHGDLPGMYPAHMYQVYKSRRGIK
RSEDSKETYKLPHRLIEKKRRDRINECIAQLKDLLPEHLKLTTLGHLEKAVVLELTLK
HVKALTNLIDQQQQKIIALQSGLQAGELSGRNVETGQEMFCSGFQTCAREVLQYLAKH
ENTRDLKSSQLVTHLHRVVSELLQGGTSRKPSDPAPKVMDFKEKPSSPAKGSEGPGKN
CVPVIQRTFAHSSGEQSGSDTDTDSGYGGESEKGDLRSEQPCFKSDHGRRFTMGERIG
AIKQESEEPPTKKNRMQLSDDEGHFTSSDLISSPFLGPHPHQPPFCLPFYLIPPSATA
YLPMLEKCWYPTSVPVLYPGLNASAAALSSFMNPDKISAPLLMPQRLPSPLPAHPSVD
SSVLLQALKPIPPLNLETKD"
BASE COUNT 322 a 375 c 311 g 231 t
ORIGIN
1 atggagcgga tccccagcgc gcaaccaccc cccgcctgcc tgcccaaagc accgggactg
61 gagcacggag acctaccagg gatgtaccct gcccacatgt accaagtgta caagtcaaga
121 cggggaataa agcggagcga ggacagcaag gagacctaca aattgccgca ccggctcatc
181 gagaaaaaga gacgtgaccg gattaacgag tgcatcgccc agctgaagga tctcctaccc
241 gaacatctca aacttacaac tttgggtcac ttggaaaaag cagtggttct tgaacttacc
301 ttgaagcatg tgaaagcact aacaaaccta attgatcagc agcagcagaa aatcattgcc
361 ctgcagagtg gtttacaagc tggtgagctg tcagggagaa atgtcgaaac aggtcaagag
421 atgttctgct caggtttcca gacatgtgcc cgggaggtgc ttcagtatct ggccaagcac
481 gagaacactc gggacctgaa gtcttcgcag cttgtcaccc acctccaccg ggtggtctcg
541 gagctgctgc agggtggtac ctccaggaag ccatcagacc cagctcccaa agtgatggac
601 ttcaaggaaa aacccagctc tccggccaaa ggttcggaag gtcctgggaa aaactgcgtg
661 ccagtcatcc agcggacttt cgctcactcg agtggggagc agagcggcag cgacacggac
721 acagacagtg gctatggagg agaatcggag aagggcgact tgcgcagtga gcagccgtgc
781 ttcaaaagtg accacggacg caggttcacg atgggagaaa ggatcggcgc aattaagcaa
841 gagtccgaag aaccccccac aaaaaagaac cggatgcagc tttcggatga tgaaggccat
901 ttcactagca gtgacctgat cagctccccg ttcctgggcc cacacccaca ccagcctcct
961 ttctgcctgc ccttctacct gatcccacct tcagcgactg cctacctgcc catgctggag
1021 aagtgctggt atcccacctc agtgccagtg ctatacccag gcctcaacgc ctctgccgca
1081 gccctctcta gcttcatgaa cccagacaag atctcggctc ccttgctcat gccccagaga
1141 ctcccttctc ccttgccagc tcatccgtcc gtcgactctt ctgtcttgct ccaagctctg
1201 aagccaatcc cccctttaaa cttagaaacc aaagattaa
//