LOCUS CR456919 684 bp mRNA linear HUM 16-OCT-2008 DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834F1115D for gene CPSF5, cleavage and polyadenylation specific factor 5, 25 kDa; complete cds, incl. stopcodon. ACCESSION CR456919 VERSION CR456919.1 KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 684) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. TITLE Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201) JOURNAL Unpublished. REFERENCE 2 (bases 1 to 684) AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld 580, D-69120 Heidelberg, Germany COMMENT RZPD; RZPDo834F1115D, ORFNo 1285 www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834F1115D RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.) RZPD LIB No. 834 www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834 www.rzpd.de/products/orfclones/ Contact: Inge Arlart RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH, Heubnerweg 6, D-14059 Berlin, Germany Tel: +49 30 32639 100 Fax: +49 30 32639 111 www.rzpd.de This clone is available from RZPD; contact RZPD (customer.service@rzpd.de) for further information. This CDS clone is a part of a collection of human full length expression clones generated by RZPD. This CDS has been cloned incl. stopcodon. The CDS has been inserted into pDONR201 via a BP Clonase(TM) reaction. Additional sequence has been added in front of the start codon: att..AAAAAA GCA GGC (ATG). The last base of the last coding triplett has been changed to T, which might lead to an amino acid change at the C terminus of the polypeptide. The stop codon has been set to TAA followed by TTAACCCAGCTTTCTT..att. Compared to the reference sequence NM_007006 we found amino acid exchange(s) at position (first base of changed triplet): 169(asp->gly) Clone distribution: http://www.rzpd.de/products/orfclones/ FEATURES Location/Qualifiers source 1..684 /db_xref="H-InvDB:HIT000267769" /organism="Homo sapiens" /lab_host="DH10B" /mol_type="mRNA" /clone_lib="Human Full ORF Clones Gateway(TM) - RZPD" /clone="RZPDo834F1115D" /note="Vector: pDONR201, Site_1: attP1; Site_2: attP2" /db_xref="taxon:9606" CDS 1..684 /codon_start=1 /gene="CPSF5" /db_xref="GOA:O43809" /db_xref="H-InvDB:HIT000267769.13" /db_xref="HGNC:HGNC:13870" /db_xref="InterPro:IPR000086" /db_xref="InterPro:IPR015797" /db_xref="InterPro:IPR016706" /db_xref="PDB:2CL3" /db_xref="PDB:2J8Q" /db_xref="PDB:3BAP" /db_xref="PDB:3BHO" /db_xref="PDB:3MDG" /db_xref="PDB:3MDI" /db_xref="PDB:3N9U" /db_xref="PDB:3P5T" /db_xref="PDB:3P6Y" /db_xref="PDB:3Q2S" /db_xref="PDB:3Q2T" /db_xref="UniProtKB/Swiss-Prot:O43809" /protein_id="CAG33200.1" /translation="MSVVPPNRSQTGWPRGVTQFGNKYIQQTKPLTLERTINLYPLTN YTFGTKEPLYEKGSSVAARFQRMREEFDKIGMRRTVEGVLIVHEHRLPHVLLLQLGTT FFKLPGGELNPGEDEVEGLKRLMTEILGRQDGVLQDWVIDDCIGNWWRPNFEPPQYPY IPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPIISSLPQL LSRFNFIYN" BASE COUNT 192 a 156 c 160 g 176 t ORIGIN 1 atgtctgtgg taccgcccaa tcgctcgcag accggctggc cccggggggt cactcagttc 61 ggcaacaagt acatccagca gacgaagccc ctcaccctgg agcgcaccat caacctgtac 121 cctcttacca attatacttt tggtacaaaa gagcccctct acgagaaggg cagctctgtt 181 gcagccagat ttcagcgcat gagggaagaa tttgataaaa ttggaatgag gaggactgta 241 gaaggggttc tgattgtaca tgagcaccgg ctaccccatg tgttactgct gcagctggga 301 acaactttct tcaaactacc tggtggtgaa cttaacccag gagaagatga agttgaagga 361 ctaaaacgct taatgacaga gatactgggt cgtcaggatg gagttttgca agactgggtc 421 attgacgatt gcattggtaa ctggtggaga ccaaattttg aacctcctca gtatccatat 481 attcctgcac atattacaaa gcctaaggaa cataagaagt tgtttctggt tcagcttcaa 541 gaaaaagcct tgtttgcagt ccctaaaaat tacaagctgg tagctgcacc attgtttgaa 601 ttgtatgaca atgcaccagg atatggaccc atcatttcta gtctccctca gctgttgagc 661 aggttcaatt ttatttacaa ttaa //