LOCUS CR456906 1059 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834F0211D for
gene EIF3S3, eukaryotic translation initiation factor 3, subunit 3
gamma, 40kDa; complete cds, incl. stopcodon.
ACCESSION CR456906
VERSION CR456906.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 1059)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 1059)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834F0211D, ORFNo 1254
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834F0211D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_003756 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..1059
/db_xref="H-InvDB:HIT000267756"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834F0211D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..1059
/codon_start=1
/gene="EIF3S3"
/db_xref="GOA:Q6IB98"
/db_xref="H-InvDB:HIT000267756.13"
/db_xref="InterPro:IPR000555"
/db_xref="InterPro:IPR027524"
/db_xref="InterPro:IPR037518"
/db_xref="UniProtKB/TrEMBL:Q6IB98"
/protein_id="CAG33187.1"
/translation="MASRKEGTGSTATSSSSTAGAAGKGKGKGGSGDSAVKQVQIDGL
VVLKIIKHYQEEGQGTEVVQGVLLGLVVEDRLEITNCFPFPQHTEDDADFDEVQYQME
MMRSLRHVNIDHLHVGWYQSTYYGSFVTRALLDSQFSYQHAIEESVVLIYDPIKTAQG
SLSLKAYRLTPKLMEVCKEKDFSPEALKKANITFEYMFEEVPIVIKNSHLINVLMWEL
EKKSAVADKHELLSLASSNHLGKNLQLLMDRVDEMSQDIVKYNTYMRNTSKQQQQKHQ
YQQRRQQENMQRQSRGEPPLPEEDLSKLFKPPQPPARMDSLLIAGQINTYCQNIKEFT
AQNLGKLFMAQALQEYNN"
BASE COUNT 329 a 247 c 241 g 242 t
ORIGIN
1 atggcgtccc gcaaggaagg taccggctct actgccacct cttccagctc caccgccggc
61 gcagcaggga aaggcaaagg caaaggcggc tcgggagatt cagccgtgaa gcaagtgcag
121 atagatggcc ttgtggtatt aaagataatc aaacattatc aagaagaagg acaaggaact
181 gaagttgttc aaggagtgct tttgggtctg gttgtagaag atcggcttga aattaccaac
241 tgctttcctt tccctcagca cacagaggat gatgctgact ttgatgaagt ccaatatcag
301 atggaaatga tgcggagcct tcgccatgta aacattgatc atcttcacgt gggctggtat
361 cagtccacat actatggctc attcgttacc cgggcactcc tggactctca gtttagttac
421 cagcatgcca ttgaagaatc tgtcgttctc atttatgatc ccataaaaac tgcccaagga
481 tctctctcac taaaggcata cagactgact cctaaactga tggaagtttg taaagaaaag
541 gatttttccc ctgaagcatt gaaaaaagca aatatcacct ttgagtacat gtttgaagaa
601 gtgccgattg taattaaaaa ttcacatctg atcaatgtcc taatgtggga acttgaaaag
661 aagtcagctg ttgcagataa acatgaattg ctcagccttg ccagcagcaa tcatttgggg
721 aagaatctac agttgctgat ggacagagtg gatgaaatga gccaagatat agttaaatac
781 aacacataca tgaggaatac tagtaaacaa cagcagcaga aacatcagta tcagcagcgt
841 cgccagcagg agaatatgca gcgccagagc cgaggagaac ccccgctccc tgaggaggac
901 ctgtccaaac tcttcaaacc accacagccg cctgccagga tggactcgct gctcattgca
961 ggccagataa acacttactg ccagaacatc aaggagttca ctgcccaaaa cttaggcaag
1021 ctcttcatgg cccaggctct tcaagaatac aacaattaa
//