LOCUS CR456904 321 bp mRNA linear HUM 03-JUN-2004
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834C057D for
gene NDUFS5, NADH dehydrogenase (ubiquinone) Fe-S protein 5, 15kDa
(NADH-coenzyme Q reductase); complete cds, incl. stopcodon.
ACCESSION CR456904
VERSION CR456904.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 321)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 321)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834C057D, ORFNo 1250
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834C057D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_004552 we did not find any
amino acid exchanges.
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..321
/db_xref="H-InvDB:HIT000267754"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834C057D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..321
/codon_start=1
/gene="NDUFS5"
/db_xref="H-InvDB:HIT000267754.14"
/db_xref="InterPro:IPR019342"
/db_xref="UniProtKB/TrEMBL:Q6IBA0"
/protein_id="CAG33185.1"
/translation="MPFLDIQKRFGLNIDRWLTIQSGEQPYKMAGRCHAFEKEWIECA
HGIGYTRAEKECKIEYDDFVECLLRQKTMRRAGTIRKQRDKLIKEGKYTPPPHHIGKG
EPRP"
BASE COUNT 100 a 64 c 89 g 68 t
ORIGIN
1 atgcctttct tggacatcca gaaaaggttc ggccttaaca tagatcgatg gttgacaatc
61 cagagtggtg aacagcccta caagatggct ggtcgatgcc atgcttttga aaaagaatgg
121 atagaatgtg cacacggaat cggttatact cgggcagaga aagagtgcaa gatagaatat
181 gatgatttcg tagagtgttt gcttcggcag aaaacgatga gacgtgcagg taccatcagg
241 aagcagcggg ataagctgat aaaggaagga aagtacaccc ctccacctca ccacattggc
301 aagggggagc ctcggcctta a
//