LOCUS CR456900 795 bp mRNA linear HUM 16-OCT-2008
DEFINITION Homo sapiens full open reading frame cDNA clone RZPDo834B047D for
gene DNAJC8, DnaJ (Hsp40) homolog, subfamily C, member 8; complete
cds, incl. stopcodon.
ACCESSION CR456900
VERSION CR456900.1
KEYWORDS Full ORF shuttle clone, Gateway(TM), complete cds.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 795)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
TITLE Cloning of human full open reading frames in Gateway(TM) system
entry vector (pDONR201)
JOURNAL Unpublished.
REFERENCE 2 (bases 1 to 795)
AUTHORS Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
JOURNAL Submitted (03-JUN-2004) to the INSDC. RZPD Deutsches
Ressourcenzentrum fuer Genomforschung GmbH, Im Neuenheimer Feld
580, D-69120 Heidelberg, Germany
COMMENT RZPD; RZPDo834B047D, ORFNo 1239
www.rzpd.de/cgi-bin/products/cl.cgi?CloneID=RZPDo834B047D
RZPDLIB; Human Full ORF Clones Gateway(TM) - RZPD (kan-resist.)
RZPD LIB No. 834
www.rzpd.de/cgi-bin/products/showLib.pl.cgi/response?libNo=834
www.rzpd.de/products/orfclones/
Contact: Inge Arlart
RZPD Deutsches Ressourcenzentrum fuer Genomforschung GmbH,
Heubnerweg 6, D-14059 Berlin, Germany
Tel: +49 30 32639 100
Fax: +49 30 32639 111
www.rzpd.de
This clone is available from RZPD;
contact RZPD (customer.service@rzpd.de) for further information.
This CDS clone is a part of a collection of human full length
expression clones generated by RZPD.
This CDS has been cloned incl. stopcodon.
The CDS has been inserted into pDONR201 via a BP Clonase(TM)
reaction.
Additional sequence has been added in front of the start codon:
att..AAAAAA GCA GGC (ATG).
The last base of the last coding triplett has been changed to T,
which might lead to an amino acid change at the C terminus of the
polypeptide.
The stop codon has been set to TAA followed by
TTAACCCAGCTTTCTT..att.
Compared to the reference sequence NM_014280 we found amino acid
exchange(s) at position (first base of changed triplet):
790(glu->asp)
Clone distribution: http://www.rzpd.de/products/orfclones/
FEATURES Location/Qualifiers
source 1..795
/db_xref="H-InvDB:HIT000267750"
/organism="Homo sapiens"
/lab_host="DH10B"
/mol_type="mRNA"
/clone_lib="Human Full ORF Clones Gateway(TM) - RZPD"
/clone="RZPDo834B047D"
/note="Vector: pDONR201, Site_1: attP1; Site_2: attP2"
/db_xref="taxon:9606"
CDS 1..795
/codon_start=1
/gene="DNAJC8"
/db_xref="GOA:O75937"
/db_xref="H-InvDB:HIT000267750.12"
/db_xref="HGNC:HGNC:15470"
/db_xref="InterPro:IPR001623"
/db_xref="InterPro:IPR036869"
/db_xref="UniProtKB/Swiss-Prot:O75937"
/protein_id="CAG33181.1"
/translation="MRGSRGGWAGEMAASGESGTSGGGGSTEEAFMTFYSEVKQIEKR
DSVLTSKNQIERLTRPGSSYFNLNPFEVLQIDPEVTDEEIKKRFRQLSILVHPDKNQD
DADRAQKAFEAVDKAYKLLLDQEQKKRALDVIQAGKEYVEHTVKERKKQLKKEGKPTI
VEEDDPELFKQAVYKQTMKLFAELEIKRKEREAKEMHERKRQREEEIEAQEKAKRERE
WQKNFEESRDGRVDSWRNFQANTKGKKEKKNRTFLRPPKVKMEQRD"
BASE COUNT 298 a 131 c 221 g 145 t
ORIGIN
1 atgagggggt cacgtggtgg ctgggccggg gaaatggcgg cttcaggaga gagcgggact
61 tcaggcggcg gaggcagcac cgaggaagca tttatgacct tctacagtga ggtgaaacaa
121 atagagaaga gagactcggt tctaacttcg aaaaatcaga ttgaaagact gacccgtcct
181 ggttcctctt acttcaattt gaacccattt gaggttcttc agatagatcc tgaagttaca
241 gatgaagaaa taaaaaagag gtttcggcag ttatccatct tggtgcatcc tgacaaaaat
301 caagatgatg ctgacagagc acaaaaggct tttgaagctg tggacaaagc ttacaagttg
361 ctactggatc aggagcaaaa gaagagggcc ctggatgtaa ttcaggcagg aaaagaatac
421 gtggaacaca ctgtgaaaga gcgaaaaaaa caattaaaga aggaaggaaa acctacaatt
481 gtagaggagg atgatcctga gctgttcaaa caagctgtat ataaacagac aatgaaactc
541 tttgcagagc tggaaattaa aaggaaagag agagaagcca aagagatgca tgaaaggaaa
601 cgacaaaggg aagaagagat tgaagctcaa gaaaaagcca aacgggaaag agagtggcag
661 aaaaactttg aggaaagtcg agatggtcgt gtggacagct ggcgaaactt ccaagccaat
721 acgaagggga agaaagagaa gaaaaatcgg accttcctga gaccaccgaa agtaaaaatg
781 gagcaacgtg attaa
//